Abstract: OBJECTIVE To investigate the metabolic characteristics of short chain fatty acids (SCFAs) in feces of infants with spleen-deficiency diarrhea. METHODS 30 children with spleen-deficiency diarrhea were selected as the diarrhea group and 30 healthy children were chosen as the control group. The short chain fatty acids in the feces were identified by gas chromatography-mass spectrometry (GC-MS). RESULTS Compared with the control group， the levels of acetic acid， propionic acid， butyric acid， isobutyric acid， valeric acid and isovaleric acid in the feces of children with spleen-deficiency diarrhea were significantly decreased. Wenyun granules significantly increased the levels of butyric acid， isobutyric acid and isovaleric acid in the feces of children with spleen-deficiency diarrhea. CONCLUSION Wenyun granules may regulate the balance of intestinal flora and improve the intestinal environment by regulating short chain fatty acids.