Abstract: OBJECTIVE To investigate the effect of Banxia Houpu Tang (BXHPT) on the model of mink vomiting induced by cisplatin and substance P/NK1R pathway. METHODS 36 minks were randomly divided into normal control group， model group， ondansetron group， BXHPT low， medium and high dose group (200， 400， 800 mg/kg). Each group was given corresponding drugs， respectively， for seven days. Then intraperitoneal injection of cisplatin was used to construct vomit model， the times of retching and vomit were measured， the content of substance P in the last area of mink brain were measured by ELISA， the protein levels of NK1R， p-ERK1/2 and ERK1/2 in the last area of the brain were measured by Western blot. RESULTS The levels of retching， vomit times， substance P， NK1R and p-ERK1/2 protein in the last area of brain in the model group were significantly higher than those in the normal control group (P<0.05). In BXHPT group， the retch， vomit times， substance P level， NK1R and p-ERK1/2 protein levels were significantly lower than those in model group (P<0.05). CONCLUSION BXHPT can treat the vomiting of mink caused by cisplatin， and the mechanism may be related to the decrease of substance P/NK1R signal activation in the last brain area of minks.