Abstract: Glycoside compounds are widely present in medicinal plants and have a variety of potential biological activities. Numerous studies have shown that sugar units in glycoside compounds play an essential role in their biological activity. Compared with traditional chemical methods， enzymatic hydrolysis by glycosylases has characterized by mild condition， environmental-friendship， and high specificity and could be used for selective hydrolysis of sugar unit. However， glycosylases from different labs suffer from various origins， uncontrollable quality and stability issues in comparison with chemical reagents. Therefore， the use of commercially available glycosylases that originate from a stable source with quality control is a feasible way to carry out selective modification on the glycoside compounds. Toward to this end， this review summarized recent applications on converting glycosides in TCM natural products by commercially available glycosidases.