Abstract: As a non-mammalian model organism， zebrafish has been widely used in the field of medical research because of its advantages of small size， easy feeding， rapid development， strong reproductive power， and easy observation. In recent years， with the continuous improvement of technology and methods， the application of model organism zebrafish in the field of traditional Chinese medicine (TCM) has also gradually deepened， especially for the evaluation and screening of complex active ingredients， active substances and toxicity of TCM. As a whole animal model， zebrafish can objectively evaluate the active substances and toxicity of TCM to achieve fast and effective high-throughput screening. In this article， by reviewing and summarizing the relevant literature at home and abroad in recent years， the application progress of model biological zebrafish in the screening of TCM activity and toxicity evaluation is reviewed， mainly from anti-inflammatory， anti-angiogenesis， pro-angiogenesis， anti-osteoporosis， Anti-alcoholic liver injury， anti-neurological disease and other active ingredients screening and hepatotoxicity and embryotoxicity are summarized and analyzed in order to provide new methods and techniques for the evaluation of the safety and effectiveness of TCM.