羌活薄层色谱和HPLC指纹图谱结合多成分含量测定的质量评价方法研究
Study on Quality Evaluation Methods of Notopterygium Incisum<\i> by HPTLC， HPLC Fingerprint and Multi-Components Quantitative Analysis
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摘要: 目的 建立薄层色谱、HPLC指纹图谱、多成分含量测定分析方法，评价18批不同产地羌活市售药材，为提高羌活质量标准提供技术支持。方法 采用高效薄层色谱法对羌活进行鉴别；采用HPLC-DAD法建立羌活指纹图谱，运用相似度软件对18批药材进行质量评价，建立HPLC法测定羌活中多个有效成分（阿魏酸苯乙醇酯、羌活醇、异欧前胡素、镰叶芹二醇）的含量。结果 建立了高效便捷的羌活薄层鉴别方法；所建立的指纹图谱出峰时间适中、方法稳定，共标定了14个共有峰，并通过对照品比对指认了6个色谱峰。18批市售药材指纹图谱相似度按共有峰计算在0.887~0.992；建立羌活多类型有效成分含量测定方法，阿魏酸苯乙醇酯、羌活醇、异欧前胡素、镰叶芹二醇在该色谱条件下分离良好，线性关系良好（r>0.999 8），平均回收率分别为95.3%、99.5%、98.5%、98.5%。结论 建立的羌活薄层鉴别方法和HPLC指纹图谱稳定可靠，能有效区别羌活、宽叶羌活及其易混淆品；同时建立了羌活中多个有效成分的HPLC含量测定方法，为全面提高羌活质量控制方法提供指导。Abstract: OBJECTIVE To establish the HPTLC identification， HPLC fingerprint and multi-components quantitative analysis methods of Notopterygium incisum (NI)， for the quality evaluation of 18 batches of NI from different origins and to provide a reference for improvement of NI quality standards. METHODS HPTLC method was applied for NI identification. HPLC-DAD method was used to establish the fingerprint of 18 batches of NI， their similarities were calculated for quality evaluation. HPLC method was performed for multiple active components (phenethyl ferulate， notopterol， isoimperatorin， falcarindiol) quantitative analysis in NI. RESULTS An efficient and convenient HPTLC method for NI identification was established. The established fingerprint had moderate running time and the method was proved to be stable. 14 common peaks were selected and 6 of them were identified by using standard substances. The similarity of 18 batches of NI was between 0.887 and 0.992. For the multi-components quantitative analysis in NI， HPLC method was established to detect phenethyl ferulate， notopterol， isoimperatorin and falcarindiol. These four peaks were separated well and had a good linear relationship (r>0.999 8) under the condition. The average recovery rates were 95.3%， 99.5%， 98.5% and 98.5%， respectively. CONCLUSION The established HPTLC and HPLC fingerprints of NI are stable and reliable， and can be used to effectively distinguish N. incisum， N. franchetii and other confusable varieties. A HPLC method for multiple active components assay is also established and validated. This study provides the guidance for comprehensively improving the quality control method of NI.
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