Abstract: OBJECTIVE To establish the HPTLC identification， HPLC fingerprint and multi-components quantitative analysis methods of Notopterygium incisum (NI)， for the quality evaluation of 18 batches of NI from different origins and to provide a reference for improvement of NI quality standards. METHODS HPTLC method was applied for NI identification. HPLC-DAD method was used to establish the fingerprint of 18 batches of NI， their similarities were calculated for quality evaluation. HPLC method was performed for multiple active components (phenethyl ferulate， notopterol， isoimperatorin， falcarindiol) quantitative analysis in NI. RESULTS An efficient and convenient HPTLC method for NI identification was established. The established fingerprint had moderate running time and the method was proved to be stable. 14 common peaks were selected and 6 of them were identified by using standard substances. The similarity of 18 batches of NI was between 0.887 and 0.992. For the multi-components quantitative analysis in NI， HPLC method was established to detect phenethyl ferulate， notopterol， isoimperatorin and falcarindiol. These four peaks were separated well and had a good linear relationship (r>0.999 8) under the condition. The average recovery rates were 95.3%， 99.5%， 98.5% and 98.5%， respectively. CONCLUSION The established HPTLC and HPLC fingerprints of NI are stable and reliable， and can be used to effectively distinguish N. incisum， N. franchetii and other confusable varieties. A HPLC method for multiple active components assay is also established and validated. This study provides the guidance for comprehensively improving the quality control method of NI.