核壳型Fe3O4@PDA@Au纳米材料对水牛角含巯基肽类成分的富集研究

刘婷, 韩疏影, 康安, 邓海山, 朱栋, 池玉梅, 刘睿

刘婷, 韩疏影, 康安, 邓海山, 朱栋, 池玉梅, 刘睿. 核壳型Fe3O4@PDA@Au纳米材料对水牛角含巯基肽类成分的富集研究[J]. 南京中医药大学学报, 2020, 36(3): 396-400.
引用本文: 刘婷, 韩疏影, 康安, 邓海山, 朱栋, 池玉梅, 刘睿. 核壳型Fe3O4@PDA@Au纳米材料对水牛角含巯基肽类成分的富集研究[J]. 南京中医药大学学报, 2020, 36(3): 396-400.
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LIUTing, HANShu-ying, KANGAn, DENGHai-shan, ZHUDong, CHIYu-mei, LIURui. Enrichment of Thiol-Containing Peptides in Water Buffalo Horn by Core-Shell Fe3O4@PDA@Au nano-Materials[J]. Journal of Nanjing University of traditional Chinese Medicine, 2020, 36(3): 396-400.
Citation: LIUTing, HANShu-ying, KANGAn, DENGHai-shan, ZHUDong, CHIYu-mei, LIURui. Enrichment of Thiol-Containing Peptides in Water Buffalo Horn by Core-Shell Fe3O4@PDA@Au nano-Materials[J]. Journal of Nanjing University of traditional Chinese Medicine, 2020, 36(3): 396-400.
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核壳型Fe3O4@PDA@Au纳米材料对水牛角含巯基肽类成分的富集研究

  • 1.

    南京中医药大学药学院，江苏 南京 210023

  • 2.

    江苏省海洋药用生物资源研究与开发重点实验室，江苏 南京 210023

  • 3.

    南京中医药大学江苏省中药资源产业化过程协同创新中心/中药资源产业化与方剂创新药物国家地方联合工程研究中心，江苏 南京 210023

Enrichment of Thiol-Containing Peptides in Water Buffalo Horn by Core-Shell Fe3O4@PDA@Au nano-Materials

  • 1.

    School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China

  • 2.

    Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing, 210023, China

  • 3.

    Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing, 210023, China

摘要

    摘要
  • 摘要: 目的 通过制备核壳型Fe3O4@PDA@Au纳米材料对水牛角中含巯基（-SH）肽类成分进行富集、鉴定与表征。方法 采用种子生长法制备核壳型Fe3O4@PDA@Au磁性纳米材料，将材料应用于水牛角提取液含-SH肽类成分的富集；分别采用Ellman法和多肽试剂盒测定富集前后溶液中-SH及多肽的含量；采用nano LC-MS/MS系统分析鉴定水牛角提取液肽类组成。结果 Fe3O4@PDA@Au纳米材料对水牛角含-SH肽类成分的富集因子为23.41，水牛角提取液中共鉴定了4 114个肽段，其中含有半胱氨酸（Cys）的肽段共11个；富集液中共鉴定了2 254个肽段，其中含有Cys的肽段共709个，富集后的含Cys的肽段与总肽段的比值从0.003增加到0.314，表明材料对水牛角提取物中含-SH肽段完成了较好的富集。结论 Fe3O4@PDA@Au纳米材料对水牛角中含巯基肽类成分具有良好的富集效果，对水牛角功效物质基础研究具有重要意义。
    Abstract: OBJECTIVE Enrichment， identification， and characterization of thiol-containing peptides in water buffalo horn by using core-shell Fe3O4@PDA@Au nano-material. METHODS The core-shell Fe3O4@PDA@Au magnetic nano-materials were prepared by seed growth method， and the material was applied to the enrichment of thiol-containing peptides in water buffalo horn extract. The contents of -SH and peptide in water buffalo horn samples were determined by Ellman method and peptide assay kit， respectively. And then the peptides in water buffalo horn extract and enriched fraction were identified and analyzed by nano LC-MS/MS system. RESULTS The enrichment factor of Fe3O4@PDA@Au nano-materials in the enrichment of thiol-containing peptides in water buffalo horn extract was calculated as 23.41. A total of 4114 peptides were identified in the water buffalo horn extract， including 11 peptides containing Cys residue; a total of 2254 peptides were identified in the enriched fraction， including 709 peptides containing Cys residue. After enrichment， the ratio of the number of thiol-containing peptide to the total peptide in water buffalo horn extract increased from 0.003 to 0.314. The results indicated that the nano-material had a high efficiency on the enrichment of thiol-containing peptides in water buffalo horn extract. CONCLUSION Fe3O4@PDA@Au nano-materials can be used for the enrichment of thiol-containing peptides in water buffalo horn， which is of great significance for the basic research of water buffalo horn.
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    • 参考文献(8)
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  • 刊出日期:  2020-05-09

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