Abstract: OBJECTIVE To establish the fingerprint and simultaneously determine the multi-characteristic components of Anemarrhenae rhizoma by HPLC-UVD-ELSD for conducting the quality analysis in the market. METHODS The chromatographic separation was conducted on an Ultimate^TM XB-C₁₈ column (4.6 mm × 250 mm， 4.5 μm)， the monitoring wavelength was 258 nm， the temperature of drift tube was maintained at 65 ℃， and the carrier gas flow rate was 1.0 L/min. The fingerprint and quantitative analysis methods of flavonoids and saponins in Anemarrhenae rhizoma were set up by HPLC-UVD-ELSD. Similarity evaluation combined with stoichiometry analysis were used to evaluate the quality of multiple batches of Anemarrhenae rhizoma samples， and multi-characteristic components in Anemarrhenae rhizoma were selected for simultaneous quantification. RESULTS An efficient and convenient HPLC-UVD-ELSD analysis method was used for fingerprint and quantitative analysis of multi-characteristic components in Anemarrhenae rhizoma. Ten main characteristic peaks were chemically identified in the established fingerprint， and the similarity ranged from 0.786 to 0.999 for 18 batches of Anemarrhenae rhizoma. The results of PLS-DA indicated that neomangiferin， timosaponin BⅡ and timosaponin BⅢ were shown greatly different in 18 batches of Anemarrhenae rhizoma samples. In addition， a quantification method of neomangiferin， mangiferin， timosaponin N， timosaponin BⅡ and timosaponin BⅢ in Anemarrhenae rhizoma was accomplished. The quantitative results of 18 batches of samples showed that the content of timosaponin B Ⅲ was the highest in Anemarrhenae rhizoma. CONCLUSION The established fingerprint and quantification of multi-characteristic components based on HPLC-UVD-ELSD analysis method can more comprehensively and accurately describe the chemical profiles and contents of characteristic compounds in Anemarrhenae rhizoma， which lays a foundation for improving effective and overall quality control method of Anemarrhenae rhizoma.