栀子苷对Treg细胞分化和功能的影响及其机制研究
Effect of Jasminoidin on Differentiation and Function of Treg Cells and Its Mechanism
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摘要: 目的 考察栀子苷对初始T细胞（Nave T）向Treg定向分化的影响和机制。方法 通过磁珠分选小鼠脾脏CD4+CD62L+T，诱导其向Treg分化，单独使用栀子苷以及与ERK通路阻断剂、JNK阻断剂合用，流式细胞术检测Treg细胞比例，Western blot法检测转录因子Foxp3、ERK、p-ERK、JNK、p-JNK等表达，ELISA法检测细胞上清IL-10含量。结果 与Control组比较，栀子苷组Treg细胞比例，Foxp3、p-ERK、p-JNK表达，IL-10含量显著增高（P<0.05~0.01）；单纯诱导的基础上先加入ERK通路阻断剂再加入栀子苷，其Treg细胞比例、Foxp3表达水平、IL-10含量显著低于栀子苷组（P<0.05~0.01），但高于Control组（P<0.01）；单纯诱导的基础上先加入JNK阻断剂再加入栀子苷，其Treg细胞比例、Foxp3表达水平、IL-10含量显著低于栀子苷组（P<0.05~0.01），但高于Control组（P<0.01）。结论 栀子苷可以显著促进Nave T向Treg定向分化，同时增强其功能，ERK、JNK可能和其发挥作用有一定关系。Abstract: OBJECTIVE To investigate the effect and mechanism of Jasminoidin on the directional differentiation of Nave T into Treg. METHODS CD4+CD62L+T was isolated from the spleen of mice by magnetic beads and induced to differentiate into Treg. Jasminoidin was used alone， and combined with ERK pathway blocker and JNK blocker. FCM was used to detect the proportion of Treg cells， and Western blot was used to detect the transcription factors of Foxp3， ERK， p-ERK，JNK and p-JNK. The expression of IL-10 were detected by ELISA method. RESULTS The percentage of Treg cells， the expression of Foxp3， p-ERK， p-JNK and the content of IL-10 in Jasminoidin group were significantly higher than those in control group when Jasminoidin was used on the basis of induction alone. On the basis of induction alone， ERK pathway blockers were added before Jasminoidin. The percentage of Treg cells， the expression of Foxp3 and the content of IL-10 were significantly lower than those of Jasminoidin group， but higher than those of control group. On the basis of induction alone， JNK blockers were added before Jasminoidin. The proportion of Treg cells， the expression of Foxp3， and the content of IL-10 were significantly lower than those of Jasminoidin group， but higher than those of control group. CONCLUSION Jasminoidin can promote the directional differentiation of Nave T into Treg and enhance its function， which may be associated with ERK and JNK.
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Keywords:
- Jasminoidin /
- Treg /
- ERK /
- JNK
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[1] 史永平， 孔浩天， 李昊楠， 等. 栀子的化学成分、药理作用研究进展及质量标志物预测分析[J]. 中草药， 2019，50(2): 281-289. [2] 俞云， 时乐， 喻斌， 等. 栀子苷对类风湿性关节炎大鼠Th17/Treg平衡和局部炎症因子的影响[J]. 南京中医药大学学报， 2018， 34(5): 73-77. [3] FANTINI MC， DOMINITZKI S， RIZZO A， et al. In vitro<\i> generation of CD4+ CD25+ regulatory cells from murine naive T cells[J]. Nat Protoc， 2007， 2(7): 1789-1794. [4] GIZINSKI AM， FOX DA. T cell subsets and their role in the pathogenesis of rheumatic disease[J]. Curr Opin Rheumatol， 2014， 26(2): 204-210. [5] 饶桂华，钮晓音.PGE_2调节CD4+T细胞分化的研究进展[J]. 现代免疫学， 2017，37(1): 55-58. [6] 李佩容，危蓉，吴志平.Th17/Treg 细胞及其相关细胞因子在溃疡性结肠炎发病中的作用及其研究进展[J]. 世界科技研究与发展， 2017， 39(2): 194-197. [7] FONTENOT JD， RASMUSSEN JP， WILLIAM LM， et al. Regulatory T cell lineage specification by the forkhead transcription factor foxp3[J]. Immunity， 2005， 22(3): 329-341. [8] WANG X， TOURNIER C. Regulation of cellular functions by the ERK5 signalling pathway[J]. Cell Signal， 2006， 18(6): 753-760. [9] WANG T， SEAH S， LOH X， et al. Simvastatin-induced breast cancer cell death and deactivation of PI3K/Akt and MAPK/ERK signalling are reversed by metabolic products of the mevalonate pathway[J]. Oncotarget， 2016， 7(3): 2532-2544. [10] RUBINFELD H， SEGER R. The ERK cascade: A prototype of MAPK signaling[J]. Mol Biotechnol， 2005， 31(2): 151-174. [11] GUPTA A， HOSSAIN MM， MILLER N， et al. NCOA3 coactivator is a transcriptional target of XBP1 and regulates PERK-eIF2alpha-ATF4 signalling in breast cancer[J]. Oncogene， 2016， 35(45): 5860-5871. [12] BOGOYEVITCH MA， KOBE B. Uses for JNK: The many and varied substrates of the c-Jun N-terminal kinases[J]. Microbiol Mol Biol Rev， 2006， 70(4): 1061-1095. [13] SABAPATHY K. Role of the JNK pathway in human diseases[J]. Prog Mol Biol Transl Sci，2012，106:145-169. -
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