Abstract: Medication in pregnancy is inevitable， and the public urgently needs accurate toxicological data to evaluate the safety of traditional Chinese medicine. Amniotic fluid can reflect the health status of the fetus as well as the mother at the same time. The change of amniotic fluid metabolism pattern is related to the pathogenesis during pregnancy. In this paper， the related researches on amniotic fluid metabolomics in fetal prediction were summarized and we took an our previous study as an example to discuss the advantages and feasibility of amniotic fluid metabolomics in the safety evaluation of Chinese medicine during pregnancy. We believe that the metabolomics of amniotic fluid， as an innovative tool with higher sensitivity and relevance， will have broad application prospects in toxicity screening and mechanism research of Chinese medicine.