Abstract: OBJECTIVE To analyze and identify the chemical constituents of Cuochuangxiao lotion. METHODS The sample was separated on an Acquity HSS T₃ reverse phase column (2.1 mm×100 mm， 1.8 μm) with the gradient elution consisted of 0.1% formic acid in MeCN and 0.1% formic acid in water as mobile phases at a flow rate of 0.3 mL/min. The electrospray ionization (ESI) source in positive or negative ion mode was used for MRM mode. The chemical constituents in Cuochuangxiao lotion were inferred and identified by accurate mass data， the element compositions analysis， the database of Scifinder， standards and relevant literatures. RESULTS 59 compounds from the Cuochuangxiao lotion were identified， mainly including anthraquinones， iridoids， organic acids and flavonoids. CONCLUSION The method is rapid and sensitive for the chemical constituent identification， which can provide the reference for clarifying the material basis and evaluation of the quality of Cuochuangxiao lotion.