Abstract: OBJECTIVE To investigate the anti-tumor effect and mechanism of Shiquan Dabu Decoction （SQDB）on mouse melanoma， and to elucidate the effect and administration of the combination with cisplatin（DDP）. METHODS Flow cytometry was used to investigate the effect of SQDB on cell cycle and apoptosis of melanoma cell line B16F10. Mouse spleen cells were isolated and MTS assay was used to investigate the anti-proliferative activity of SQDB in spleen cells induced by ConA or LPS. The model of mouse melanoma subcutaneous xenograft was established. The anti-tumor effect of SQDB was detected by HE staining， immunohistochemistry and TUNEL staining. The melanoma metastasis model was constructed to study the potential mechanism on the inhibition of the tumor metastasis by SQDB. RESULTS 10 mg/mL SQDB could induce apoptosis of B16F10 melanoma cells; 2.5， 5， 10 mg/mL SQDB reduced the number of G₀/G₁ cells; 10 mg/mL SQDB increased the number of S phase cells; 2.5 mg/mL of SQDB increased the number of cells in G₂/M phase; 5% and 10% drug-containing serum promoted the proliferation of B lymphocytes induced by LPS; 5%， 10% and 15% drug-containing serum also promoted the proliferation of T lymphocytes caused by Con A. Using DDP and SQDB simultaneously or using DDP first and then SQDB inhibited the growth of melanoma. Using both DDP and SQDB significantly inhibited tumor cell proliferation and promoted tumor cell apoptosis; When DDP and SQDB were used together， or when DDP was used first， SQDB could significantly inhibit melanoma lung metastasis. In addition， the thymus and spleen coefficient of the mice decreased significantly when DDP alone was used (P<0.05， P<0.01)， and the simultaneous administration of SQDB and DDP could increase the thymus coefficient of the mice (P<0.05). CONCLUSION SQDB can inhibit the proliferation of melanoma B16F10， promote B16F10 apoptosis and the proliferation of mouse spleen lymphocytes. Combined with DDP， SQDB can enhance the anti-tumor effect of mouse xenografts and metastatic tumor models.