Abstract: OBJECTIVE Based on glycosyltransferase β3GnT8， to investigate the mechanism of reverse effect on triple negative breast cancer MDA-MB-231 drug-resistance cells by Zhuidu Fang. METHODS The inhibition of MDA-MB-31 drug-resistance cells proliferation by Zhuidu Fang was detected by MTT assay. By interference and transfection of β3GnT8， high and low expressed β3GnT8 MDA-MB-231/TAX cell lines were obtained. Western blot was used to detect the protein expression of β3GnT8， and drug resistance protein BCRP and P-gp. Lectin flow cytometry was used to detect β3GnT8 fluorescence intensity on β3GnT8 transfected MDA-MB-231/TAX cells. RESULTS Zhuidu Fang effectively inhibited the proliferation of MDA-MB-231/TAX cells， and the inhibitory rate was 50.16% after 72 h. Zhuidu Fang significantly downregulated the protein expression of β3GnT8， BCRP and P-gp. The protein expression of β3GnT8 showed a positive correlation with BCRP and P-gp. CONCLUSION Zhuidu Fang can reverse the drug-resistance of MDA-MB-231 cell lines， which is associated with the downregulation of the expression of β3GnT8， BCRP and P-gp.