越鞠甘麦大枣汤调节小鼠海马PKA-CREB-BDNF信号通路的抗抑郁作用

Antidepressant-Like Effects of Yueju Ganmai Dazao Decoction Associated with PKA-CREB-BDNF Signaling Pathway in the Hippocampus of Mice

    摘要
  • 摘要: 目的 探讨越鞠甘麦大枣汤（YG）的抗抑郁功效及其与PKA-CREB-BDNF信号通路的关联。方法 强迫游泳（FST）检测越鞠甘麦大枣汤单次给药后抗抑郁的时效性。慢性不可预测模型（CMS）进一步检测越鞠甘麦大枣汤在抑郁模型上的抗抑郁作用。蛋白印迹表达法检测越鞠甘麦大枣汤单次给药30 min和24 h后海马中PKA、CREB、BDNF的蛋白表达。结果 YG在FST中显示出持久的抗抑郁潜力，并且快速逆转了慢性应激小鼠的抑郁样行为。在慢性小鼠模型中，海马中PKA，CREB和BDNF的表达显著上调。结论 越鞠甘麦大枣汤（YG）显示出快速和持久的抗抑郁样作用，其抗抑郁功能与PKA-CREB-BNDF通路有关。

     

    Abstract: OBJECTIVE To explore the antidepressant effect of Yueju Ganmai Dazao Decoction (YG) and its association with PKA-CREB-BDNF signaling pathway. METHODS Forced swimming (FST) was used to detect the time-effect of anti-depression after a single administration of YG.The chronic unpredictable model (CMS) further tested the antidepressant effect of YG on the depression model.Western blot expression was used to detect the expression of PKA， CREB and BDNF in hippocampus at 30 min and 24 h after single administration of YG. RESULTS YG showed long-lasting antidepressant potential in FST， meanwhile it also significantly and rapidly reversed behavioral deficits in chronically stressed mice. In the chronic mouse model， the expression of PKA， CREB and BDNF in the hippocampus was significantly up-regulated. CONCLUSION YG displays rapid and lasting antidepressant-like effect and its function is related to the PKA-CREB-BNDF pathway.

     

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