栀子活性部位GJ-4的HPLC指纹图谱及主成分含量测定研究

Study on HPLC Fingerprint and Content Determination of Major Costituents of GJ-4，an Effective Fraction from Gardeniae Fructus

    摘要
  • 摘要: 目的 建立GJ-4 HPLC指纹图谱，并明确特征色谱峰的化学成分，测定主含有效成分藏红花苷-3的含量。方法 采用Phenomenex Gemini C18色谱柱(250 mm×4.6 mm，5 μm)，以乙腈-0.1%甲酸水为流动相进行梯度洗脱，检测波长440 nm，建立GJ-4的HPLC指纹图谱，通过相似度软件对其进行相似度分析；对主要有效成分藏红花苷-3进行含量测定。结果 建立了GJ-4的HPLC指纹图谱，对17个主要特征峰进行明确的化学指认，20批GJ-4的指纹图谱相似度在0.939~0.994之间，藏红花苷-3的含量在0.200~0.683 mg/g之间。结论 该方法专属性强，准确度高，可作为GJ-4质量控制的有效方法之一。

     

    Abstract: OBJECTIVE To establish a HPLC fingerprint of GJ-4 (an effective fraction from Gardeniae Fructus) and to quantify the main component crocin-3. METHODS The experiment was carried out on a Phenomenex Gemini C18 column (250 mm×4.6 mm， 5 μm)， eluted with ACN-water with 1‰ formic gradiently and determined at 440 nm wavelength. HPLC fingerprint chromatogram of GJ-4 was set up and quality of 20 batches of GJ-4 was evaluated by similarity assay. The major contents of crocin-3 was determined. RESULTS The HPLC fingerprint chromatogram of GJ-4 was been established， in which 17 compounds were identified. The similarity of 20 batches of GJ-4 was about 0.939 to 0.994， and the content of crocin-3 was about 0.200 to 0.682 mg/g. CONCLUSION The method established in this paper can provide a comprehensive reference for the quality control of GJ-4.

     

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