Abstract: OBJECTIVE To investigate the relationship between the inhibiting expression of inflammatory factors and improvement on insulin resistance treated by Sanhuang Tang. METHODS C57BL/6 male mice were respectively fed with high fat diet and normal diet for 12 weeks. HFD (High fat diet) mice were divided randomly to DIO model group， Sanhuang Tang treatment and metformin treatment group. Normal mice fed with normal diet were set as control group. The inflammatory factors expression in serum were examined by milliplex assay. The number of macrophages and CD8+ T cells in epididymis adipose tissue was examined by FACS. RESULTS Compared with DIO model group， Sanhuang Tang treatment significantly decreased the HOMA-IR factor， and slightly decreased fasting blood glucose and fasting insulin. At the same time， Sanhuang Tang significantly decrease the expression of TNF-α， IL-1β， MCP-1，resistin compared with model group. Sanhuang Tang significantly decrease the number of macrophages in epididymis adipose tissue， while did not significantly alter the number of CD8+ T cells. CONCLUSION Sanhuang Tang may improve insulin resistance induced by obesity in HFD mice by inhibiting macrophages infiltration and the expression of inflammatory factors.