麸炒苍术不同提取物对小鼠脾虚泄泻的影响
Effect of Bran Stir-Baked Atractylodis Rhizoma Water Extract， Ethanol Extract and CH2Cl2 Active Parts on Spleen-Deficiency Diarrhea in Mice
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摘要: 探究麸炒苍术水提物、醇提物、二氯甲烷部位对脾虚泄泻模型小鼠的治疗作用。方法 选用番泻叶水煎液灌胃制备大鼠脾虚模型。考察麸炒苍术水提物、醇提物、二氯甲烷萃取物对脾虚小鼠体质量、腹泻等级、粪便含水率、血清淀粉酶、D-木糖水平及肠内短链脂肪酸（SCFAs）含量的影响，并做肠道病理学进行观察。结果 与模型组比较，麸炒苍术水提物、醇提物、二氯甲烷部位均可显著减轻腹泻等级、缓解脾虚泻引起的体质量增长迟缓，降低粪便含水率，增加血清D-木糖及肠内短链脂肪酸含量，减轻肠黏膜损伤，此外麸炒苍术二氯甲烷部位组血清淀粉酶水平较模型组显著提高。结论 麸炒苍术水提物、醇提物、二氯甲烷部位均能明显拮抗番泻叶所致小鼠脾虚泻，麸炒苍术二氯甲烷部位对于脾虚泻引起消化吸收功能降低能起到最好的改善作用。Abstract: OBJECTIVE Study of bran stir-baked Atractylodis Rhizoma water extract，ethanol extract and CH2Cl2 active parts on Efficacy with Spleen-deficiency Diarrhea(SDD) in mice. METHODS The spleen deficiency mouse models were established by extract of Sennae Folium and treated by bran stir-baked Atractylodis Rhizoma water extract， ethanol extract and CH2Cl2 active parts.Body weight，diarrhea index， fecal moisture，levels of serum amylase，D-xylose in serum and short chain fatty acids (SCFAs) contents in cecum of mice were studied， and intestinal pathology examination were observed. RESULTS Compared with model group， the body weights， the levels of D-xylose and SCFAs contents in the water extract， ethanol extract and CH2Cl2 active parts from bran stir-baked Atractylodis Rhizoma obviously increased. but the levels of diarrhea index， fecal moisture and relieve intestinal mucosal injury were significantly decreased. moreover，the level of serum amylase in the CH2Cl2 active parts group increased compared with model group. CONCLUSION The water extract，ethanol extract and CH2Cl2 active parts from bran stir-baked Atractylodis Rhizoma have obvious anti diarrhea effect in mice caused by extract of Sennae Folium. CH2Cl2 active parts show best improvement in digestion and absorption function.
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