基于NF-κB、RANK探讨七味通痹口服液对类风湿关节炎大鼠的影响

The Effect on Rheumatoid Arthritis Rats of Qiwei Tongbi Oral Liquid Based on NF-κB and RANK

    摘要
  • 摘要: 研究七味通痹口服液（QWTB）对胶原诱导关节炎(Collagen-induced arthritis，CIA)大鼠模型的治疗作用及其机制。方法 大鼠右侧足趾和尾根部皮下多点注射弗氏完全佐剂和Ⅱ型胶原混合物复制大鼠CIA模型，成模后随机分为模型组，QWTB 12.20、6.10、3.05g/kg3个剂量组和醋酸地塞米松（0.893mg/kg）组（地米组），另设空白对照组。21d后，在尾根部皮下注射进行激发免疫，14d后处死。观察QWTB对大鼠足趾容积，继发病变，血清中TNF-α、IL-1α、IL-17水平及关节滑膜NF-κB、RANK、RANKL蛋白表达的影响。结果 QWTB3个剂量均可不同程度地抑制关节炎大鼠足趾肿胀度，减轻继发病变，降低炎性细胞因子和滑膜NF-κB、RANK、RANKL蛋白表达，其中12.20、6.10g/kg剂量组与模型组比较均有显著性差异（P<0.05~0.01）。结论 QWTB可以通过下调炎性细胞因子水平，抑制骨损伤，减轻CIA大鼠关节病变。

     

    Abstract: OBJECTIVE To investigate the therapeutic effect and mechanism of Qiwei Tongbi(QWTB) oral liquid on the rat model of collagen-induced arthritis (CIA)． METHODS The mixture of Freund's complete adjuvant and type Ⅱ collagen was injected into the right toe and tail root with subcutaneous multipoint method to copy the CIA rat model. Successful model rats were randomly divided into model group， QWTB 12.20， 6.10， 3.05g/kg groups and dexamethasone acetate 0.893mg/kg group， respectively. Saline solution was injected into control group rats (n=10). After 21d， rheumatoid arthritis was stimulated by the tail root subcutaneous injection， and the rats were executed after 14d. Rats toes volume， the secondary lesion， inflammation score， the level of TNF-α， IL-1α and IL-17， and the protein expression of NF-κB， RANK and RANKL in the synovium of joint were examined to determine the effect of QWTB on CIA rats. RESULTS Three doses of QWTB inhibited the swelling degree of the arthritis in rats toes， reduced the secondary lesion， and decreased the inflammation score and the levels of inflammatory cytokines. High dose group and middle dose group showed significant differences， compared with model group (P<0.05， P<0.01）. CONCLUSION QWTB can decrease the levels of inflammatory cytokines to alleviate joint injury of CIA rats.

     

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