胶态二氧化硅与β-环糊精固化丁香油比较研究
Comparative Study on Colloidal Silica Dioxide and β-Cyclodextrin Solidifying Clove Volatil Oil
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摘要: 目的 比较β-环糊精和胶态二氧化硅SYLOID 244FP 2种不同方法固化丁香油的特性。方法 以β-环糊精和胶态二氧化硅为载体，分别用饱和水溶液法和研磨法固化丁香油制备固化粉末，采用HPLC法测定丁香酚含量并比较两者的收率，采用差示扫描量热法（DSC）比较两者固化粉末的物相表征，采用加速试验法考察两者的稳定性。结果 β-环糊精固化粉末中丁香酚的平均收率为78.59%，胶态二氧化硅固化粉末中丁香酚的平均收率为98.69%；2种固化粉末DSC图中的丁香油吸热峰均消失，得到了较好的包合；丁香油、β-环糊精固化粉末和胶态二氧化硅固化粉末在60 ℃下密闭放置8 h加速试验中丁香酚损失率分别为17.35%、1.76%和3.74%，敞口放置8 h加速试验中丁香酚损失率分别为24.29%、4.64%和5.97%。结论 胶态二氧化硅和β-环糊精均能较好地固化丁香油，但前者辅料用量少，操作简单，丁香油收率明显高于后者，具有较高的研究价值和潜在的应用前景。Abstract: OBJECTIVE To compare the characteristics of two different methods that solidified clove volatile oil (CVO) using β-cyclodextrin and colloidal silicon dioxide SYLOID 244FP. METHODS The CVO-solidified powder was prepared by saturated aqueous solution method and grinding method using β-cyclodextrin and colloidal silicon dioxide as carrier. The content of eugenol was determined by HPLC and the yield was compared. The structures of the solidified powder was characterized by differential scanning calorimetry (DSC). The stability of the two kinds of solidified powder was investigated by accelerated test. RESULTS The average yield of eugenol in the β-cyclodextrin solidified powder and in colloidal silicon dioxide solidified powder was 78.59% and 98.69%， respectively. The endothermic peak of the two kinds of solidified powder was disappeared in the phase of DSC. The loss ratios of eugenol in clove volatile oil， β-cyclodextrin solidified powder and colloidal silicon dioxide solidified powder were 17.35%， 1.76% and 3.74%， respectively at 60 ℃ for 8 h， while eugenol loss ratios were 24.29%， 4.64% and 5.97% in an open state. CONCLUSION Both colloidal silicon dioxide and β-cyclodextrin can solidify clove volatile oil， but the former needs less excipient， and preparation process with higher clove volatil oil yield， which shows high research value and potential application prospect.
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