黄芩苷对辐射诱导的小鼠急性肺损伤保护作用机制研究

葛永亮; 陶金华; 吴木军

黄芩苷对辐射诱导的小鼠急性肺损伤保护作用机制研究

1.
南通市通州区人民医院影像科，江苏南通 226000
2.
南通大学药学院，江苏南通 226001

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- 刊出日期: 2017-11-09

Effect of Baicalin on Radiation-Induced Acute Lung Injury in Mice Through Antioxidant and Anti-Inflammatory Properties

1.
Department of Radiology, Tongzhou District People's Hospital, Nantong, 226000, China
2.
School of Pharmacy, Nantong University, Nantong, 226001, China

摘要

摘要: 目的探讨黄芩苷对辐射诱导小鼠的急性肺损伤保护作用。方法 X-线复制小鼠的急性肺损动物模型。将50只ICR小鼠随机分为空白对照组、X-线组、X-线组+地塞米松组（5mg/kg）、X-线组+黄芩苷组（20、40mg/kg）。观察各组肺组织病理学改变，测量肺湿/干质量比，支气管肺泡灌洗液中性粒细胞百分比、肺组织中超氧化物歧化酶（SOD）、丙二醛（MDA）、炎症因子白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）含量及肺组织中p-NFκBp65、p-IκBα蛋白表达。结果黄芩苷组（20、40mg/kg）可有效减轻辐射所致肺组织病理学变化，能降低肺泡灌洗液中炎症细胞的数目，能显著降低肺湿/干质量比重，能提高SOD水平，并能降低MDA水平，降低p-NFκBP65和p-IκBα蛋白的表达。结论黄芩苷可减轻辐射所致急性肺组织损伤，对辐射诱导的急性肺损伤有保护作用。
- 黄芩苷 /
- 辐射 /
- 急性肺损伤 /
- NF-κBp65
Abstract: OBJECTIVE The current study was designed to evaluate the protective effects of baicalin on radiation-induced acute lung injury in mice. METHODS 50 ICR mice were divided into five groups randomly， namely， control group， X-ray group， X-ray+dexamethasone group (5mg/kg)， X-ray+baicalin group (20， 40 mg/kg)， with 8 in each group. Pathological changes in the lung tissues were observed. The ratio of lung wet/dry weight was also recorded. Besides， the level of neutrophil in bronchoalveolar lavage fluid (BALF) was detected to evaluate inflammatory cell infiltration. Moreover， the expressions of MDA， IL-6， TNF-α， p-NFκBp65 and p-IκBα， as well as the activity of SOD， were examined in lung. RESULTS Baicalin (20， 40 mg/kg) could effectively improve the radiation-induced lung pathological changes and reduce the number of inflammatory cells in BALF. The ratio of lung wet/dry weight was also decreased with the treatment of baicalin (20， 40 mg/kg). In addition， the activity of SOD was recovered and the end product of lipid peroxidation MDA was reduced meanwhile. The expressions of p-NFκBp65 and p-IκBα was extensively down-regulated with baicalin (20， 40 mg/kg) preconditioning， taking the expressions of NFκBp65 and IκBα as inner controls. CONCLUSION Baicalin could alleviate radiation-induced acute lung injury due to its antioxidant and anti-inflammatory properties.
- baicalin /
- radiation /
- acute lung injury /
- NF-κBp65

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文章访问数: 1118
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黄芩苷对辐射诱导的小鼠急性肺损伤保护作用机制研究

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出版历程

Effect of Baicalin on Radiation-Induced Acute Lung Injury in Mice Through Antioxidant and Anti-Inflammatory Properties

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其他类型引用(8)

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