拨法对CCI大鼠脊髓中IL-6及SOCS3的影响
Effects of BoFa on Expression of IL-6 and SOCS3 in Spinal Cord in CCI Rats
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摘要: 目的 从炎症反应的角度探索拨法对神经病理性疼痛大鼠的影响。方法 采用推拿手法模拟仪定性、定量模拟拨法，对CCI大鼠进行干预，通过光热耐痛阈观察大鼠行为学的改善情况，通过免疫组化观察大鼠脊髓中IL-6及SOCS3表达的变化。结果 造模7d后，模型组大鼠光热耐痛阈结果与正常组相比有显著差异（P<0.01），模型组大鼠脊髓中IL-6及SOCS3的表达较正常组显著升高（P<0.01）；拨法组用拨法治疗20次后，大鼠光热耐痛阈结果与同期模型组相比有显著差异（P<0.05），且逐渐趋向于正常水平，大鼠脊髓中IL-6的表达较同期模型组有显著降低（P<0.01），脊髓中SOCS3的表达较同期模型组有显著性升高（P<0.01）。结论 拨法可一定程度上改善神经病理性疼痛大鼠的痛觉和温度觉，同时可抑制大鼠体内促炎症因子的表达。Abstract: OBJECTIVE To explore the effect of BoFa for neuropathic pain in rats from the perspective of inflammatory reaction. METHODS The CCI model rats were intervened qualitatively and quantitatively by the massage simulation instrument. Observe the variation of rats' behavior though solar-thermal pain threshold resistance. Observe the variation of IL-6 and SOCS3 in spinal cord in rats through Immunohistochemical technique. RESULTS After seven days of surgery， the results of solar-thermal pain threshold resistance of the model group were significance changed than that of normal group(P<0.01)， the results of IL-6 and SOCS3 in spinal cord in rats were significantly increased than that of normal group(P<0.01). After twenty times of BoFa therapy， the results of solar-thermal pain threshold resistance of the BoFa group were significance changed than that of model group(P<0.05)， and near normal group level; the results of IL-6 in spinal cord in rats were significantly reduce than that of model group(P<0.01); the results of SOCS3 in spinal cord in rats were significantly increased than that of model group(P<0.01). CONCLUSION BoFa can improve the pain sense and temperature sense of CCI rats in a certain extent， and it also can inhibit the expression of pro-inflammatory factors in rats.
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Keywords:
- neuropathic pain /
- BoFa /
- CCI /
- IL-6 /
- SOCS3
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