拨法对CCI大鼠脊髓中IL-6及SOCS3的影响

马驰, 姚斌彬, 于天源, 陶艳红, 鲁梦倩, 贾文端, 张林峰, 郭鑫

马驰, 姚斌彬, 于天源, 陶艳红, 鲁梦倩, 贾文端, 张林峰, 郭鑫. 拨法对CCI大鼠脊髓中IL-6及SOCS3的影响[J]. 南京中医药大学学报, 2017, 33(4): 399-402.
引用本文: 马驰, 姚斌彬, 于天源, 陶艳红, 鲁梦倩, 贾文端, 张林峰, 郭鑫. 拨法对CCI大鼠脊髓中IL-6及SOCS3的影响[J]. 南京中医药大学学报, 2017, 33(4): 399-402.
MA Chi, YAO Bin-bin, YU Tian-yuan, TAO Yan-hong, LU Meng-qian, JIA Wen-duan, ZHANG Lin-feng, GUO Xin. Effects of BoFa on Expression of IL-6 and SOCS3 in Spinal Cord in CCI Rats[J]. Journal of Nanjing University of traditional Chinese Medicine, 2017, 33(4): 399-402.
Citation: MA Chi, YAO Bin-bin, YU Tian-yuan, TAO Yan-hong, LU Meng-qian, JIA Wen-duan, ZHANG Lin-feng, GUO Xin. Effects of BoFa on Expression of IL-6 and SOCS3 in Spinal Cord in CCI Rats[J]. Journal of Nanjing University of traditional Chinese Medicine, 2017, 33(4): 399-402.

拨法对CCI大鼠脊髓中IL-6及SOCS3的影响

Effects of BoFa on Expression of IL-6 and SOCS3 in Spinal Cord in CCI Rats

  • 摘要: 目的 从炎症反应的角度探索拨法对神经病理性疼痛大鼠的影响。方法 采用推拿手法模拟仪定性、定量模拟拨法,对CCI大鼠进行干预,通过光热耐痛阈观察大鼠行为学的改善情况,通过免疫组化观察大鼠脊髓中IL-6及SOCS3表达的变化。结果 造模7d后,模型组大鼠光热耐痛阈结果与正常组相比有显著差异(P<0.01),模型组大鼠脊髓中IL-6及SOCS3的表达较正常组显著升高(P<0.01);拨法组用拨法治疗20次后,大鼠光热耐痛阈结果与同期模型组相比有显著差异(P<0.05),且逐渐趋向于正常水平,大鼠脊髓中IL-6的表达较同期模型组有显著降低(P<0.01),脊髓中SOCS3的表达较同期模型组有显著性升高(P<0.01)。结论 拨法可一定程度上改善神经病理性疼痛大鼠的痛觉和温度觉,同时可抑制大鼠体内促炎症因子的表达。
    Abstract: OBJECTIVE To explore the effect of BoFa for neuropathic pain in rats from the perspective of inflammatory reaction. METHODS The CCI model rats were intervened qualitatively and quantitatively by the massage simulation instrument. Observe the variation of rats' behavior though solar-thermal pain threshold resistance. Observe the variation of IL-6 and SOCS3 in spinal cord in rats through Immunohistochemical technique. RESULTS After seven days of surgery, the results of solar-thermal pain threshold resistance of the model group were significance changed than that of normal group(P<0.01), the results of IL-6 and SOCS3 in spinal cord in rats were significantly increased than that of normal group(P<0.01). After twenty times of BoFa therapy, the results of solar-thermal pain threshold resistance of the BoFa group were significance changed than that of model group(P<0.05), and near normal group level; the results of IL-6 in spinal cord in rats were significantly reduce than that of model group(P<0.01); the results of SOCS3 in spinal cord in rats were significantly increased than that of model group(P<0.01). CONCLUSION BoFa can improve the pain sense and temperature sense of CCI rats in a certain extent, and it also can inhibit the expression of pro-inflammatory factors in rats.
  • [1] 马翔宇. 白细胞介素-17(IL-17)正向调节星形胶质细胞IL-6正反馈表达及其调控机理的研究[D].济南:山东大学,2010.
    [2] MA XY. Interleukin-17 (IL-17) Enhancement of the Positive Feedback Loop of IL-6 in Astrocytes[D]. Jinan: Shandong University,2010.
    [3] 王志福,俞向梅,龚德贵,等. 电针“阳陵泉”、“环跳”对神经病理性疼痛大鼠脊髓SOCS3的影响[J]. 中国疼痛医学杂志,2015,21(5):335-340.
    [4] WANG ZF, YU XM, GONG DG, et al. Effects of electroacupuncture at "Yanglinquan" and "Huantiao" acupoints on expression of SOCS3 in spinal cord in rats with neuropathic pain[J]. Chin J Pain Med, 2015,21(5):335-340.
    [5] VALLEJO R, TILLEY DM, VOGEL L, et al. The role of Glia and the immune system in the development and maintenance of neuropathic pain[J]. Pain Pract,2010,103.
    [6] HEYDENDAEL W,JACOBSON L. Widespread hypothalamic-pi-tuitary-adrenocortical axis-relevent and mood-relevant effects of chronic fluoxetine treatment on glucocorticoid receptor gene expression in mice[J].Eur J Neurosci,2010,31(5):892-902.
    [7] 于天源,林彩霞,付国兵,等.按摩推拿学[M].第3版.北京:中国协和医科大学出版社,2012:19.
    [8] YU TY, LIN CX, FU GB, et al. Massage[M]. 3rd edition. Beijing: Beijing union medical university press,2012:19.
    [9] CROKER BA, KREBS DL, ZHANG JG, et al. SOCS3 negatively regulates IL-6 signaling in vivo[J]. Nat Immunol,2003,4(6):40-45.
    [10] DOMINGGUEZ E, MAUBORGNE A, MALLET J, et al. SOCS3-mediated blockade of JAK/STAT3 signaling pathway reveals its major contribution to spinal cord neuroinflammation and mechanical allodynia after peripheral nerve injury[J]. J Neurosci,2014,25,273:65-78.
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出版历程
  • 收稿日期:  2016-12-24
  • 修回日期:  2017-03-28
  • 刊出日期:  2017-07-09

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