Abstract: OBJECTIVE Taking dregs of Ginkgo Folium from ginkgo diterpene lactone meglumine injection production process as material， to optimize the extraction technology of total flavonoids and polyprenols. METHODS The L₉ (3) orthogonal test method was carried out to investigate the effects of pre-leaching time， extraction time， extraction temperature and particle sieving on the effect of polypentenols extract by petroleum ether. Ethanol concentration， solid-liquid ratio， extraction time and extraction time on the extraction efficiency of total flavonoids were also investigated. The optimal comprehensive extraction process of polyprenols and total flavonoids in the dregs of Ginkgo Folium was chosen. RESULTS The dregs of Ginkgo Folium was crushed and sieved through 50 mesh， then soaked in petoleum ether for 0.5h， extracted by reflux at 40℃ for 1h twice. The filtrates were combined and concentrated without ether flavor to be polypentenols extract. The dregs after petroleum ether treatment were dried. 20 times of 95% ethanol were added and extracted by reflux for 0.5h twice. The filtrates were combined and dried to be dry extract as total flavonoids extract. CONCLUSION The method was simple and easy for the extraction of polyprenols and total flavonoids from dregs of Ginkgo Folium after water extracting， which provides a reference for the utilization efficiency and industrial production.