人参平肺方对二氧化硅所致大鼠肺纤维化的作用及机制探讨
The Effect and Mechanism of Renshen Pingfei Prescription in the Pulmonary Fibrosis Model Induced by Silica in Rats
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摘要: 目的 在二氧化硅粉尘悬液致大鼠纤维化模型上观察人参平肺方的作用并探讨其可能机制。方法 SD大鼠麻醉后在气管内注入二氧化硅粉尘悬液以建立肺纤维化模型，然后随机分为模型组、泼尼松组(5.0mg/kg)、人参平肺方3.0、6.0、12.0g/kg剂量组，同时另设空白对照组。连续给药48d后取大鼠肺组织测定肺系数，HE及Masson染色观察肺泡炎症及纤维化程度，测试盒检测肺组织中羟脯氨酸（HYP）含量，ELISA法检测肺泡灌洗液TGF-β1、NF-κB、PDGF、TNF-α及IFN-γ水平，免疫组织化学染色检测肺组织TGF-β1、Samd2/3及ERK1/2蛋白的表达。结果 人参平肺方能够显著降低肺纤维化大鼠的肺系数（P<0.05），降低肺泡炎症及纤维化程度，降低肺泡灌洗液TGF-β1、NF-κB、PDGF及TNF-α水平（P<0.05），升高肺泡灌洗液IFN-γ水平（P<0.05），降低肺组织TGF-β1、Samd2/3及ERK1/2的蛋白表达（P<0.05）。结论 人参平肺方对肺纤维化大鼠有治疗作用，其机制可能与TGF-β/Smad/ERK信号通路有关。Abstract: OBJECTIVE To research the effect of Renshen Pingfei prescription in the pulmonary fibrosis model induced by Silica in rat and discuss the possible mechanism. METHODS SD rats were anesthetized and injected with silica dust suspension into trachea to establish pulmonary fibrosis model，then randomly divided into model group， prednisone group， Renshen Pingfei prescription 3.0，6.0，12.0g/kg dose group， and establish normal group. Every day on time， the intragastric administration were proceeded for 48 days and lung tissue of rats were weighed to calculate pulmonary coefficient. Evaluating the inflammation and fibrosis of pulmonary alveoli through HE and Masson HE staining.The content of hydroxyproline（HYP） in lung tissue was detected by assay kit.The level of TGF-β1， NF-κB， PDGF， TNF-α and IFN-γ in alveolar lavage fluid were detected using ELISA-kit and the protein expression level of TGF-β1， Samd2/3 and ERK1/2 in lung were detected through immunohistochemical staining. RESULTS The kidney coefficient， the inflammation and fibrosis of pulmonary alveoli， the level of TGF-β1， NF-κB， PDGF， and TNF-α in alveolar lavage fluid the protein expression level of TGF-β1， Samd2/3 and ERK1/2 in lung were significantly decreased（P<0.05）in the pulmonary fibrosis model of rat after administration with Renshen Pingfei prescription， otherwise the level of IFN-γ in alveolar lavage fluid were significantly increased（P<0.05）. CONCLUSION Renshen Pingfei prescription has protective effect in pulmonary fibrosis and its mechanism may relate to TGF-β/Smad/ERK signal.
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Keywords:
- pulmonary fibrosis /
- Renshen Pingfei prescription /
- Silica /
- TGF-β1
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