苦瓜多糖的纯化、结构解析及其免疫调节和抗肿瘤活性研究
Purification， Structural Characterization and Immunomodulatory and Antitumor Activities of a Polysaccharide Isolated from Momordica Charantia L.
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摘要: 目的 提取并纯化苦瓜多糖，对其结构特征进行解析并研究其免疫调节和抗肿瘤活性。方法 热水提取苦瓜多糖，透析后纤维素阴离子交换色谱和凝胶过滤层析纯化得到多糖产物。利用高效凝胶渗透色谱法测定多糖分子量，并采用FT-IR和NMR法对其局部结构特征进行解析。利用细胞分子生物学技术和药理学等手段测定苦瓜多糖的免疫调节作用和抗肿瘤活性。结果 分离纯化获得分子量为745 kDa的杂多糖MCP-2，测定其单糖组成为鼠李糖、半乳糖醛酸、半乳糖、木糖和阿拉伯糖，对应的摩尔比为1.19∶15.97∶3.40∶0.73∶0.94。此外，还发现MCP-2能够促进正常小鼠淋巴细胞增殖，提高巨噬细胞RAW264.7中炎症因子NO、IL-1β和TNF-α的表达量。MCP-2还具有体内抑制S180肿瘤细胞的活性。结论 本研究表明MCP-2具有显著的免疫增强和肿瘤抑制的活性。Abstract: OBJECTIVE To isolate and purify an active polysaccharide of Momordica charantia L. and investigate its structural characterization and immunomodulatory and antitumor activities. METHODS A polysaccharides was isolated from Momordica charantia L. in hot water extraction followed by dialysis and purification through anion exchange cellulose chromatography and gel filtration chromatography， and its molecular weight was measured by high performance gel permeation chromatography. Additionally， Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance (NMR) were used to determine its partial structural characterization， and its immunomodulatory and antitumor activities were measured by cell and molecular biological techniques and pharmacological methods. RESULTS A heteropolysaccharide named MCP-2 with molecular weight of 745 kDa was isolated and purified， which was shown to be consisted of rhamnose， galacturonic acid， galactose， xylose， and arabinose with corresponding molar ratio being 1.19∶15.97∶3.40∶0.73∶0.94. Furthermore， MCP-2 was found to promote the lymphocyte proliferation of normal mice and to raise the levels of NO， IL-1β and TNF-α produced by macrophage RAW264.7. Intriguingly， MCP-2 presented the antitumor activity against S180 cells in vivo as well. CONCLUSION All experimental data showed that MCP-2 had apparent immunologic enhancement and antitumor activities.
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期刊类型引用(14)
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