苦瓜多糖的纯化、结构解析及其免疫调节和抗肿瘤活性研究

张逸, 王旺, 蔡寅, 王旻, 顾春艳, 杨烨

张逸, 王旺, 蔡寅, 王旻, 顾春艳, 杨烨. 苦瓜多糖的纯化、结构解析及其免疫调节和抗肿瘤活性研究[J]. 南京中医药大学学报, 2017, 33(1): 33-39.
引用本文: 张逸, 王旺, 蔡寅, 王旻, 顾春艳, 杨烨. 苦瓜多糖的纯化、结构解析及其免疫调节和抗肿瘤活性研究[J]. 南京中医药大学学报, 2017, 33(1): 33-39.
ZHANG Yi, WANG Wang, CAI Yin, WANG Min, GU Chun-yan, YANG Ye. Purification, Structural Characterization and Immunomodulatory and Antitumor Activities of a Polysaccharide Isolated from Momordica Charantia L.[J]. Journal of Nanjing University of traditional Chinese Medicine, 2017, 33(1): 33-39.
Citation: ZHANG Yi, WANG Wang, CAI Yin, WANG Min, GU Chun-yan, YANG Ye. Purification, Structural Characterization and Immunomodulatory and Antitumor Activities of a Polysaccharide Isolated from Momordica Charantia L.[J]. Journal of Nanjing University of traditional Chinese Medicine, 2017, 33(1): 33-39.

苦瓜多糖的纯化、结构解析及其免疫调节和抗肿瘤活性研究

Purification, Structural Characterization and Immunomodulatory and Antitumor Activities of a Polysaccharide Isolated from Momordica Charantia L.

  • 摘要: 目的 提取并纯化苦瓜多糖,对其结构特征进行解析并研究其免疫调节和抗肿瘤活性。方法 热水提取苦瓜多糖,透析后纤维素阴离子交换色谱和凝胶过滤层析纯化得到多糖产物。利用高效凝胶渗透色谱法测定多糖分子量,并采用FT-IR和NMR法对其局部结构特征进行解析。利用细胞分子生物学技术和药理学等手段测定苦瓜多糖的免疫调节作用和抗肿瘤活性。结果 分离纯化获得分子量为745 kDa的杂多糖MCP-2,测定其单糖组成为鼠李糖、半乳糖醛酸、半乳糖、木糖和阿拉伯糖,对应的摩尔比为1.19∶15.97∶3.40∶0.73∶0.94。此外,还发现MCP-2能够促进正常小鼠淋巴细胞增殖,提高巨噬细胞RAW264.7中炎症因子NO、IL-1β和TNF-α的表达量。MCP-2还具有体内抑制S180肿瘤细胞的活性。结论 本研究表明MCP-2具有显著的免疫增强和肿瘤抑制的活性。
    Abstract: OBJECTIVE To isolate and purify an active polysaccharide of Momordica charantia L. and investigate its structural characterization and immunomodulatory and antitumor activities. METHODS A polysaccharides was isolated from Momordica charantia L. in hot water extraction followed by dialysis and purification through anion exchange cellulose chromatography and gel filtration chromatography, and its molecular weight was measured by high performance gel permeation chromatography. Additionally, Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance (NMR) were used to determine its partial structural characterization, and its immunomodulatory and antitumor activities were measured by cell and molecular biological techniques and pharmacological methods. RESULTS A heteropolysaccharide named MCP-2 with molecular weight of 745 kDa was isolated and purified, which was shown to be consisted of rhamnose, galacturonic acid, galactose, xylose, and arabinose with corresponding molar ratio being 1.19∶15.97∶3.40∶0.73∶0.94. Furthermore, MCP-2 was found to promote the lymphocyte proliferation of normal mice and to raise the levels of NO, IL-1β and TNF-α produced by macrophage RAW264.7. Intriguingly, MCP-2 presented the antitumor activity against S180 cells in vivo as well. CONCLUSION All experimental data showed that MCP-2 had apparent immunologic enhancement and antitumor activities.
  • 期刊类型引用(14)

    1. 彭兴兴,林丽霞,彭飞飞,吴颖茹,陈晓丽,罗珊. 苦瓜化学成分及其功能特性研究进展. 食品安全导刊. 2023(29): 182-185 . 百度学术
    2. 阿尔祖古丽·阿卜力米提,李敬双,金鑫,于洋. 苦瓜多糖对小鼠淋巴细胞免疫调节作用的影响. 粮油食品科技. 2022(01): 105-112 . 百度学术
    3. 谷俊华,肖付才. 基于热风干燥温度的苦瓜片干燥动力学及其品质探究. 开封大学学报. 2022(01): 87-93 . 百度学术
    4. 王亚军,张文煊,李宇清. 苦瓜皂苷的急性毒性及解热抗炎作用研究. 药学研究. 2020(03): 130-132+137 . 百度学术
    5. 赖婷. 苦瓜中主要活性物质及其生理功能的研究进展. 食品安全导刊. 2020(09): 87+95 . 百度学术
    6. 周潇恬,罗非君. 苦瓜的功能成分和生物活性研究进展. 现代食品. 2020(10): 66-71 . 百度学术
    7. 王恒禹,罗云凤,姜京华,丁侃,董艳萍. 玉米须多糖的提取纯化及结构研究. 首都师范大学学报(自然科学版). 2019(01): 44-49 . 百度学术
    8. 袁华,陈尚锋,李辉. 苦瓜多糖对人乳腺癌MDA-MB-231细胞生物学行为的影响. 临床和实验医学杂志. 2019(22): 2392-2397 . 百度学术
    9. 王铖博,陈乐乐,焦彩珍,石继鹏,慕星星,张继. 陕甘花楸果实多糖的结构表征及抗氧化活性研究. 广西植物. 2019(11): 1527-1533 . 百度学术
    10. 张晓寒,张程慧,于文睿,薛婉茹,佟彤,孙嘉璘,冯叙桥. 药食同源类植物多糖降血糖功效的研究进展. 食品安全质量检测学报. 2018(14): 3699-3705 . 百度学术
    11. 帕丽达·阿不力孜,米仁沙·牙库甫,陈橙,丛媛媛. 胀果甘草多糖GiP-3的结构分析及免疫活性测定. 中国实验方剂学杂志. 2018(04): 66-71 . 百度学术
    12. 雍文兴,颜春鲁,安方玉,伍志伟,苏韫,雒志义,朱俊燚. 当归多糖对染镉大鼠肾氧化损伤和Bcl-2/Bax表达的影响. 中医研究. 2018(03): 71-75 . 百度学术
    13. Xiaohan ZHANG,Chenghui ZHANG,Wenrui YU,Wanru XUE,Tong TONG,Jialin SUN,Xuqiao FENG. Advances in Research on Hypoglycemic Functions of Polysaccharides from Medicinal and Edible Plants. Medicinal Plant. 2018(06): 1-6 . 必应学术
    14. 李海霞,张伟. 红景天多糖对宫颈癌U14模型小鼠的治疗作用及机制的实验研究. 中药材. 2017(06): 1453-1456 . 百度学术

    其他类型引用(12)

计量
  • 文章访问数:  2062
  • HTML全文浏览量:  13
  • PDF下载量:  1613
  • 被引次数: 26
出版历程
  • 刊出日期:  2017-01-09

目录

    /

    返回文章
    返回