黄芩苷对溃疡性结肠炎模型大鼠NF-κB表达的影响
Effect of Baicalin on Expression of NF-κB in Ulcerative Colitis Rats
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摘要: 目的 观察黄芩苷对溃疡性结肠炎模型大鼠核因子-κB（Nuclear factor-κB，NF-κB）表达及下游炎症细胞因子的影响。方法 采用TNBS法制备大鼠溃疡性结肠炎模型，随机分为正常组、模型组、黄芩苷（Baicalin）高中低剂量组、阳性对照组（柳氮磺胺吡啶，SASP组）。给药10 d后，留取结肠组织标本，观察结肠大体形态变化，采用ELISA法观察UC模型大鼠结肠组织中肿瘤坏死因子（TNF-α）、白介素-8（IL-8）和白介素-1（IL-1）的含量，采用Western blot法检测NF-κB的表达水平。结果 与模型组相比，黄芩苷100 mg/kg组能显著改善结肠大体形态，黄芩苷（25、50、100 mg/kg）干预后能剂量依赖性的抑制各炎症因子的分泌，黄芩苷高剂量组能降低IL-1、IL-8和TNF-α表达水平，但与SASP组比较无明显差异。黄芩苷高剂量组能降低NF-κB的水平，促进IκBα水平（P<0.05）。结论 黄芩苷能够抑制NF-κB的活化，从而发挥其在溃疡性结肠炎中的抗炎效应。Abstract: OBJECTIVE To explore the effect of Baicalin in vivo of the expression of NF-κB and cytokines in ulcerative colitis rats. METHODS The colitis model of mice were established with TNBS methord.Mice were randomly divided into control group，model group，Baicalin group，and SASP group.After continuous administration for 10 days，observed the colon gross morphological changes，the colon tissue level of TNF-α，IL-8 and IL-1 were detected using ELISA， and the protein expression of NF-κB in the colon tissues of mice were detected using Western Blot. RESULTS Compared with the model group，Baicalin(100 mg/kg) group could significantly improve the colon gross morphological form，the Baicalin(25，50，100 mg/kg) groups could inhibit the secretion of inflammatory factors dose-dependently， the high dose group could reduce IL-1，IL-8 and TNF-αexpression (P<0.05)， no significant difference occured between the SASP group. Compared with the model group，.Baicalin high dose group could reduce NF-kB levels， promote IkBα level (P<0.05) . CONCLUSION Baicalin can inhibit NF-κB activation， and thus play its anti-inflammatory effect in ulcerative colitis mice.
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