皮炎洗剂对小鼠慢性变应性接触性皮炎的影响及其作用机制
Study of the Effect and Mechanism of Dermatitis Lotion in the Treatment of Allergic Contact Dermatitis in Mice
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摘要: 目的 评价皮炎洗剂治疗小鼠慢性变应性接触性皮炎（ACD）的疗效。方法 采用2，4-二硝基氯苯（DNCB）诱导小鼠背部ACD。60只小鼠随机分为空白对照组、ACD组、阳性组、皮炎洗剂大剂量组(0.4g/mL)、中剂量组(0.2g/mL)、小剂量组(0.1g/mL)，每组10只，于致敏后给药，观察各组小鼠第4次激发24h后皮损炎症程度、组织病理切片、炎症细胞数量改变及血清中IFN-γ和IL-4水平的变化。结果 皮炎洗剂中、大剂量能有效减轻ACD小鼠引起的免疫器官肿大、皮损炎症程度，减少皮损组织炎症细胞数量，调节ACD组小鼠血清中γ-干扰素(IFN-γ)和白细胞介素-4(IL-4)水平。结论 皮炎洗剂对小鼠ACD具有明显的治疗作用，其作用机制可能与皮炎洗剂调节Th1、Th2型细胞因子有关。
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关键词:
- 皮炎洗剂 /
- 小鼠 /
- 慢性变应性接触性皮炎 /
- IFN-γ /
- IL-4
Abstract: OBJECTIVE To study the efficacy of dermatitis lotion in the treatment of allergic contact dermatitis in mice. METHODS The 2，4-dinitro chlorobenzene(DNCB) was applied on the dorsum of mice to induce the allergic contact dermatitis models. 60 mice were randomly divided into control， model(ACD)， momestasone furoate， dermatitis lotion (0.4， 0.2， 0.1g/mL) groups， with 10 mice in each group. Mice were sensitized by DNCB and then treated topically with different drugs on lesions. The inflammation degree of skin lesion were observed， histopathological analysis was measured by HE strain and the levels of serum IFN-γ and IL-4 were detected by ELISA. RESULTS The dermatitis lotion of 0.4g/mL restrained the increase of thymus index， alleviated the inflammation degree of lesion on dorsum of mice and reduced the pathological score significantly， compared with the model group. In addition， the dermatitis lotion of 0.4g/mL adjusted the expression of serum IFN-γ and IL-4 to return to normal levels. CONCLUSION These results suggest that dermatitis lotion can be used for the treatment of allergic contact dermatitis (ACD) mainly through the regulation Th1/Th2 cytokines.-
Keywords:
- dermatitis lotion /
- mice /
- allergic contact dermatitis /
- IFN-γ /
- IL-4
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[1] Yuan XY， Liu W， Zhang P， et al. Effects and mechanisms of aloperine on 2，4-dinitrofluorobenzene-induced allergic contact dermatitis in BALB/c mice[J]. Eur J Pharmacol， 2010， 10(1/3): 147-152. [2] 陈妍，郭在培．变应性接触性皮炎的原位免疫学研究[J]．华西医学，2006， 21（1）：217-218． [3] Chen Y， Guo ZP. In situ immunology research of allergic contact dermatitis[J]. West China Med J， 2006， 21(1): 217-218. [4] Harskamp CT， Armstrong AW. Immunology of atopic dermatitis: novel insights into mechanisms and immunomodulatory therapies[J]. Semin Cutan Med Surg， 2013， 32(3): 132-139. [5] Yoon HJ， Jang MS， Kim HW， et al. Protective effect of diet supplemented with rice prolamin extract against DNCB-induced atopic dermatitis in BALB/c mice[J]. BMC Complement Altern Med， 2015， 15: 353. [6] Baeck M， Soria A， Marot L， et al. Characterization of the T cell response in allergic contact dermatitis caused by corticosteroids[J]. Contact Dermatitis， 2013， 68(6): 357-368. [7] 梁秀宇．消风散对变应性接触性皮炎的抑制作用及其免疫学机制的实验研究[D]．沈阳：辽宁中医药大学，2007． [8] Liang XY. The Experimental Study on Xiaofeng Powder in the Inhibiting Function and the Immunology Mechanism to Allergy Contact Dermatitis[D]．Shenyang：Liaoning University of Chinese Medicine，2007． [9] Yamashita-Kanemaru Y， Takahashi Y. CD155(PVR/Nec15) mediates a costimulatory signal in CD4+T cells and regulates allergic inflammation[J]. J Immunol， 2015， 194(12): 5644-5653. [10] Pennino D， Eyerich K， Scarponi C， et al. IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes[J]. J Immunol， 2010， 184(9): 4880-4888. [11] Choi JK， Oh HM， Lee S， et al. Oleanolic acid acetate inhibits atopic dermatitis and allergic contact dermatitis in a murine model[J]. Toxicol Appl Pharmacol， 2013， 269(1): 72-80. [12] 芦源．养血消风饮对变应性接触性皮炎的作用及免疫学机制研究[D]．沈阳：辽宁中医药大学，2009． [13] Lu Y. The Effects and Immunological Mechanisms of Yangxue Xiaofeng Yin on Allergic Contact Dermatitis[D]．Shenyang：Liaoning University of Chinese Medicine，2009．
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