Abstract: OBJECTIVE To study the efficacy of dermatitis lotion in the treatment of allergic contact dermatitis in mice. METHODS The 2，4-dinitro chlorobenzene(DNCB) was applied on the dorsum of mice to induce the allergic contact dermatitis models. 60 mice were randomly divided into control， model(ACD)， momestasone furoate， dermatitis lotion (0.4， 0.2， 0.1g/mL) groups， with 10 mice in each group. Mice were sensitized by DNCB and then treated topically with different drugs on lesions. The inflammation degree of skin lesion were observed， histopathological analysis was measured by HE strain and the levels of serum IFN-γ and IL-4 were detected by ELISA. RESULTS The dermatitis lotion of 0.4g/mL restrained the increase of thymus index， alleviated the inflammation degree of lesion on dorsum of mice and reduced the pathological score significantly， compared with the model group. In addition， the dermatitis lotion of 0.4g/mL adjusted the expression of serum IFN-γ and IL-4 to return to normal levels. CONCLUSION These results suggest that dermatitis lotion can be used for the treatment of allergic contact dermatitis (ACD) mainly through the regulation Th1/Th2 cytokines.