Abstract: OBJECTIVE To investigate the effects of Whenshen Zhixie Fang administrated through bellybutton and the influence on 5-HT and NO of spleen and kidney yang deficiency type IBS-D patients. METHODS By using computer， parallel group and non-blinded trials， patients were randomly divided into two groups. 58 cases in the treatment group were given Wenshen Zhixie Fang administrated through bellybutton together with orally taken Montmorillonite powder. The control group was given orally taken Montmorillonite powder only. The treating effects were assessed after ten days. RESULTS Total effective rate of the treatment group was 82.76%， which was better than that of the control group (66.10%)(P<0.05). Abdominal pain and discomfort were significantly relieved in the treatment group， and the shape of the stool and frequency were also changed for better (P<0.01)， which were better than those of the control group except the stool. And 5-HT was significantly decreased and NO was increased， which were better than those of the control group(P<0.01).CONCLUSION Wenshen Zhixie Fang administered through Bellybutton can improve the clinical symptoms of IBS-D and its mechanism may be related to the regulation of 5-HT and NO levels and reduction of intestinal sensitivity.