Abstract: OBJECTIVE To compare the contents of three kinds of flavonoids (scutellarin， baicalin and wogonoside) and total flavonoids in different organs of Scutellaria baicalensis Georgi (root， stem， leave anf flower)， medicinal herbs of Scutellaria(tender Scutellaria， old Scutellaria and green Scutellaria) and its callus. METHODS SYMMETRY C₁₈（4.6 mm×250 mm，5 μm） column was used with the solvent system of acetonitrile (A)-0.22% acetic acid water (B) and the UV detection wavelength of 335 nm， at the temperature of 35 ℃， to detect the contents of three kinds of flavonoids. The contents of total flavonoids were determined with the control sample of rutin， the indicators of aluminum nitrate and the UV detection wavelength of 504 nm. RESULTS The linear ranges of scutellarin， baicalin and wogonoside were 6.0~192.0， 30.0~960.0， 12.0~384.0 μg/mL（R²≤0.998 9）， respectively. The average recoveries were 97.8%~103.1%， RSD≤4.0%. The linear ranges of total flavonoids was 40.0~240.0 mg/mL（R²≥0.999 8）， and the average recoveries was 98.8%(RSD=1.0%) . CONCLUSION The content of scutellarin in aboveground Scutellaria baicalensis Georgi (flower， stems and leaves) was higher than underground part (root)， and the content in flowers is highest， reaching the point of 0.53%. Baicalin and wogonoside content in underground part of are higher than aboveground， and the content in callus baicalin reaches 0.60%. In callus， the content of total flavonoid is highest (30.42%). Those results may provide a basis for scutellarin extraction from Scutellaria flower and the production of total flavonoids from Scutellaria callus.