Abstract: OBJECTIVE To investigate the effects of component formula of Salviae miltiorrhizae radix et rhizome and Ginseng panax et rhizome on cell migration， invasion and phosphorylation of ERK1/2 in lung cancer A549 cells. METHODS The scratch wound healing assay and high content screening were used to analyze the effects of component formula of Salviae Miltiorrhizae Radix Et Rhizoma and Ginseng Radix Et Rhizome on A549 cell migration. The Real-time cell analysis was adapted to detect the effects of component formula on A549 cell invasion. The phosphorylation of ERK1/2 in lung cancer A549 cells was measured by nanoscale ultramicro protein analysis system. RESULTS Component formula treatment significantly increased the distance of scratch blank area in time-dependent manner(P<0.01)， compared with control group; decreased A549 cells migration area(P<0.01) in a dose-dependent pattern; significantly inhibited A549 cells invasion(P<0.01) in time-dependent manner; and decreased the phosphorylation of ERK1/2(P<0.01) in A549 cells. CONCLUSION The component formula of Salviae Miltiorrhizae Radix Et Rhizome and Ginseng Panax Et Rhizome can inhibit A549 cells migration and invasion， which may be related to the decreased phosphorylation level of ERK1/2.