针刺对2型糖尿病大鼠胰岛素抵抗及胰岛β细胞形态学的影响
Effects of Acupuncture Oninsulin Resistance and Morphologic Changes of Islet β-cell in Rat with Type 2 Diabetes Mellitus
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摘要: 目的 观察“调理脾胃针法”对2型糖尿病大鼠胰岛素抵抗及胰岛β细胞形态学的影响。方法 健康雄性Wistar大鼠36只，随机抽取10只作为空白组，并将剩余大鼠采用高脂高糖饲料联合小剂量（25 mg/kg）腹腔注射链脲佐菌素的方式制作2型糖尿病大鼠模型，而后将造模成功20只大鼠采用分层随机的方法分为针刺组10只、模型组10只，其中空白组大鼠不做任何干预；针刺组大鼠着针刺服后采用“调理脾胃针法”干预，连续针刺4周；模型组大鼠只采用相同方法抓取、穿衣，而不作干预。各组大鼠于干预前及干预后每周末测量体质量、空腹血糖、餐后2 h血糖以及干预前后测量空腹血清胰岛素含量及胰岛素敏感指数，并于末次干预后处死大鼠，摘取胰腺，做HE染色，观察胰岛结构。结果 针刺1周后，针刺组大鼠体质量仍明显高于空白组（P<0.05），而模型组却与空白组大鼠体质量无明显差异（P>0.05）。针刺第4周末，模型组体质量均显著低于针刺组、空白组（P<0.05），而针刺组与空白组比较，体质量无明显差异（P>0.05）；与模型组比较，针刺组空腹血糖及餐后2 h血糖于针刺1周后开始显著降低（P<0.01），且针刺2、3、4周后，空腹血糖及餐后2 h血糖均仍有持续显著降低（P<0.01）；干预前针刺组与模型组大鼠空腹血清胰岛素均明显高于空白组（P<0.01），且胰岛素敏感指数明显低于空白组（P<0.01）。干预后模型组空腹血清胰岛素水平均显著高于针刺组与空白组，而针刺组与空白组比较无明显差异（P>0.05）。与模型组比较，针刺组胰岛素敏感指数显著提高（P<0.01）。胰岛HE染色则显示干预后针刺组形态学改变优于模型组，并接近于空白组。结论 “调理脾胃针法”可有效降低大鼠空腹及餐后2 h血糖，并可降低空腹血清胰岛素水平，提高胰岛素敏感性，改善胰岛素抵抗现象，维持糖尿病大鼠体质量，以及促进胰岛结构及胰岛β细胞修复。Abstract: OBJECTIVE To study the effects of Regulating spleen-stomach on acupuncture on insulin resistance (IR) and morphologic changes of islet β-cell in rat with type 2 diabetes mellitus (T2DM). METHODS 10 rats were randomly selected into normal group from thirty-six male Wistar clean-grade rats. The rest rats were made The T2DM rat model，which established with streptozotocin(25 mg/kg) and high fat diet. Twenty rats were made T2DM rat model successfully，which divided into acupuncture group (10 rats)and model group(10 rats)，according to Stratified randomization. Normal group were not given any treatment. Acupuncture group were treated with acupuncture that were dressed in clothes which reduce restless.and the treatment was given for continuous 4 weeks.But model group were not given any treatment. Several outcome indicators were measured such as body mass， fasting blood-glucose(FBG)， 2 hour postprandial blood glucose(P2BG)， fasting insulin(FINS)， insulin sensitivity index(ISI).After the last intervention，the rats were killed and pancreas were made HE staining. RESULTS One weeks after the intervention， body mass of acupuncture group increased significantly compared with normal group（P<0.05），which had no significantly difference with model group（P>0.05）. Body mass of model group were significantly reduced compared with normal group and acupuncture group at the four weeks after the intervention（P<0.05）. The FBG and P2BG of acupuncture group were lower than model group at the every weeks after the intervention（P<0.01）.FINS of model group and acupuncture group were higher than normal group front the intervention（P<0.01），which increased significantly compared with other groups in the ISI（P<0.01）. FINS of model group were higher than acupuncture group and normal group after the intervention（P<0.01），what is more，ISI of acupuncture group increased significantly compared with model group（P<0.05）. Acupuncture group has protective effects of morphology of islet β-cell. CONCLUSION Regulating spleen-stomach on acupuncture could reduces the level of FBG， P2BG and FINS and improves ISI and relieve IR， which maintains rats of body mass promoting restoration of islet and β-cell.
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