电针对中枢Stat5敲除所致肥胖小鼠瘦素水平和下丘脑Kctd15、Sh2b1 mRNA的影响
Effects of Electro-acupuncture on Serum Leptin Content and Expression of Kctd15 and Sh2b1 mRNA in Hypothalamus in Stat5-knockout Induced Obese Mice
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摘要: 目的 观察电针对中枢Stat5条件性敲除Stat5 Nestin-Cre (Stat5NKO)肥胖小鼠下丘脑Kctd15、Sh2b1基因表达的影响。方法 20只肥胖的Stat5NKO小鼠随机分为肥胖组和电针组,每组10只;10只Stat5fl/fl小鼠作为正常组。其中电针组选取单侧后三里、内庭穴进行针刺治疗,每日1次,双侧交替进行,每次30 min,韩氏电针仪频率2/15 Hz,疏密波,6 d为1个疗程,共治疗4个疗程。运用ELISA法检测血清中瘦素(Leptin)含量,qPCR技术检测下丘脑中肥胖相关基因Kctd15、Sh2b1 mRNA的表达。结果 与正常组小鼠比较,肥胖组小鼠体质量明显增加(P<0.01)伴随血清Leptin含量升高(P<0.05),下丘脑Leptin水平下降(P<0.05),同时下丘脑Kctd15、Sh2b1 mRNA表达下调(P<0.05~0.01);与肥胖组小鼠比较,电针组小鼠的体质量明显下降(P<0.01),血清Leptin含量降低(P<0.01),且下丘脑Leptin水平回升(P<0.01),Kctd15、Sh2b1 mRNA表达水平上调(P<0.01)。结论 电针可能通过调节Leptin水平并促进Kctd15、Sh2b1表达,实现对Stat5NKO小鼠的减肥效应。Abstract: OBJECTIVE To observe the effect of electro-acupuncture on central conditional knockout Stat5 Nestin-Cre obese mice (Stat5NKO) and detect how the obesity related genes, Kctd15 and Sh2b1, are involved. METHODS 20 obese Stat5NKO mice were randomly divided into two groups: obese model group (n=10) and electro-acupuncture (EA) group (n=10); 10 Stat5fl/fl mice were randomly selected and fed with ordinary forage as normal control group. EA group was treated with electro-acupuncture at Housanli and Neiting points for 30 min, with bilateral alternates, by Han' electro-acupuncture apparatus at 2/15 Hz. 6 d was a period of treatment, with a total of 4 courses of treatment. Serum Leptin content was measured by ELISA, and the levels of Sh2b1 and Kctd15 mRNA in hypothalamus were detected by qPCR. RESULTS Compared with Stat5fl/fl mice, Stat5NKO mice gained body weight significantly (P<0.01), accompanied with increased serum Leptin level (P<0.05), decreased hypothalamic Leptin level (P<0.05), as well as Kctd15 and Sh2b1 mRNA expressions (P<0.05~0.01); Compared with Stat5NKO mice, EA group decreased body weight (P<0.01) and serum Leptin mRNA level (P<0.01). The hypothalamic Leptin level was elevated (P<0.01), and the expressions of Kctd15 and Sh2b1 mRNA were returned to normal level (P<0.01). CONCLUSION The weight-loss effect of electro-acupuncture on Stat5NKO obese mice may be related to the regulation of the expression of Leptin, Kctd15 and Sh2b1.
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Keywords:
- obesity /
- electro-acupuncture /
- Leptin /
- Kctd15 /
- Sh2b1
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[1] Cho SH, Lee JS, Thabane L, et al. Acupuncture for obesity: a systematic review and meta-analysis[J]. Int J Obes (Lond), 2009, 33(2): 183-196. [2] 于月,尤佳,杨柳,等.瘦素信号转导通路在能量代谢平衡中的作用机制[J].生命科学,2013, 25(2):169-175. [3] Yu Y, You J, Yang L, et al. Leptin receptor signaling in energy homeostasis[J]. Chin Bull Life Sci, 2013, 25(2): 169-175. [4] Shen W, Wang Y, Lu SF, et al. Acupuncture promotes white adipose tissue browning by inducing UCP1 expression on DIO mice[J]. BMC Complement Altern Med, 2014, 14: 501. [5] Lee JY, Muenzberg H, Gavrilova O, et al. Loss of cytokine-STAT5 signaling in the CNS and pituitary gland alters energy balance and leads to obesity[J]. PLoS One, 2008, 3(2): e1639. [6] Myers J, Olson DP. Central nervous system control of metabolism[J]. Nature, 2012, 491(7424): 357-363. [7] Zarelli VE, Dawid IB. Inhibition of neural crest formation by Kctd15 involves regulation of transcription factor AP-2[J]. Proc Natl Acad Sci USA, 2013, 110(8): 2870-2875. [8] Cao H, Sekiya M, Ertunc ME, et al. Adipocyte lipid chaperone AP2 is a secreted adipokine regulating hepatic glucose production[J]. Cell Metab, 2013, 17(5): 768-778. [9] De Lartigue G, Ronveaux CC, Raybould HE. Deletion of leptin signaling in vagal afferent neurons results in hyperphagia and obesity[J]. Mol Metab, 2014, 3(6): 595-607.
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