Abstract: OBJECTIVE To study the optimum preparation condition of inclusion complexes of resveratrol by ultrasonic method. METHODS The inclusion complexes were prepared by ultrasonic method. The optimum preparation condition was studied by orthogonal test with encapsulation rate as the index of evaluation. RESULTS The optimum preparation condition：the ratio of resveratrol to β-CD is 1∶2，inclusion temperature is 50℃，ultrasonic time is 4 hours，ultrasonic power was 300W. The inclusion of inclusion rate is 44.58%. CONCLUSION The inclusion complexes prepared by ultrasonic method have such advantages:simplicity of operator， condition easily controled，slightly affected by external influence.