电针大肠俞募穴对功能性便秘小鼠结肠组织GDNF表达的影响

张微; 李瑛; 刘丽莎; 罗芳丽; 郑倩华

电针大肠俞募穴对功能性便秘小鼠结肠组织GDNF表达的影响

成都中医药大学针灸推拿学院，四川成都 610072

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- 收稿日期: 2015-01-05
- 修回日期: 2015-05-21
- 刊出日期: 2015-09-09

Effects of Electroacupuncture at Back-shu and Front-mu Points on Expression of GDNF in Colon Tissue of Functional Constipation Mice

摘要

摘要: 目的探讨电针大肠俞募穴治疗功能性便秘的作用机制。方法 32只小鼠随机分为对照组、模型组、针刺组和EGC抑制组。采用复方地芬诺酯灌胃造模法，电针刺激天枢、大肠俞，每次30 min，共治疗5次。运用光镜、电镜、免疫组化及原位杂交技术，观察电针治疗前后结肠组织上皮细胞形态学改变，结肠组织中GDNF蛋白及mRNA表达水平。结果与对照组比较，模型组结肠组织GDNF蛋白及mRNA表达水平显著降低（P<0.01）。电针刺激大肠俞募穴可提高结肠中GDNF蛋白及其mRNA的表达水平（P<0.05），光镜和电镜发现针刺可修复受损上皮细胞，进而改善肠道传输功能。EGC抑制组结肠中GDNF蛋白表达与模型组比较无显著性差异（P>0.05），与针刺组比较显著降低（P<0.05），GDNF mRNA的表达水平高于模型组（P<0.05），低于针刺组，但差异无统计学意义（P>0.05）。光镜和电镜下EGC抑制组结肠组织形态改变较为严重。结论电针刺激大肠俞募穴可提高EGC细胞中GDNF蛋白及其mRNA的表达水平，修复受损结肠上皮细胞，进而改善肠道传输功能。
- 电针 /
- 俞募穴 /
- 功能性便秘 /
- GDNF
Abstract: OBJECTIVE To explore the mechanism of electroacupuncture at Back-shu and Front-mu points for functional constipation treatment. METHODS 32 mice were randomly divided into control group， model group， electroacupuncture (EA) group and EGC group. Compound diphenoxylate were gavaged for modeling， and electroacupuncture Tianshu and Dachangshu points， 30 min each time for a total of 5 times treatment. By light microscopy， electron microscopy， immunohistochemistry and in situ hybridization， the morphology of colonic epithelial structure were observed， and the level of GDNF protein and mRNA expression in colon were detected. RESULTS The expression of GDNF protein and mRNA in model group was significantly decreased (P<0.01). Electroacupuncture at back-Shu and front-Mu points improved the expression of GDNF protein and mRNA (P<0.05)， and light microscope and electron microscope showed that acupuncture repaired damaged depithelial cells， and then improved the intestinal transmission function. The expression of GDNF protein in EGC group showed no significant difference with the model group (P>0.05)， but significantly decreased， compared with EA group (P<0.05). GDNF mRNA was significantly higher than model group (P<0.05)， but lower than EA group with no significant difference (P>0.05). The morphology change of colon tissue is more serious. CONCLUSION Electroacupuncture at back-Shu and front-Mu points can improve the expression of GDNF protein and mRNA in EGC cells， repair the colonic epithelial cells， and then improve the intestinal transmission function.
- electroacupuncture /
- Back-shu and Front-mu points /
- functional constipation /
- GDNF

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参考文献(27)

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电针大肠俞募穴对功能性便秘小鼠结肠组织GDNF表达的影响

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出版历程

Effects of Electroacupuncture at Back-shu and Front-mu Points on Expression of GDNF in Colon Tissue of Functional Constipation Mice

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