三黄煎剂调节Aurora激酶A促进乳腺癌细胞凋亡的实验研究

许岩磊; 陈曦琰; 陈绪; 卞卫和; 姚昶; 朱小纾; 陈佳静

三黄煎剂调节Aurora激酶A促进乳腺癌细胞凋亡的实验研究

1.
南京中医药大学附属医院，江苏南京 210029
2.
西悉尼大学生物和健康学院，澳大利亚 2750

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出版历程
- 收稿日期: 2015-05-02
- 修回日期: 2015-08-10
- 刊出日期: 2015-09-09

San Huang Decoction Promotes Apoptosis of Breast Cancer Cells Through Regulating Aurora Kinase A

1.
The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China
2.
The School of Science and Health, The University of West Sydney, 2750, Australia

摘要

摘要: 目的探讨三黄煎剂对乳腺癌MCF-7、MDA-MB-231细胞凋亡的影响及Aurora激酶A（Aurora A）蛋白表达及功能的影响，并探讨其内在机制。方法采用CCK-8法检测三黄煎剂对乳腺癌细胞MCF-7、MDA-MB-231增殖的影响。AnnexinV-FITC/PI法检测MCF-7、MDA-MB-231细胞凋亡率。q-PCR法检测MCF-7、MDA-MB-231细胞Aurora A、p53的mRNA表达水平。Western Blot法检测MCF-7、MDA-MB-231细胞凋亡相关蛋白的表达及Aurora A蛋白的表达。结果三黄煎剂对MCF-7、MDA-MB-231细胞增殖抑制率呈浓度梯度依赖增长（P<0.05），给药48 h疗效好于24 h（P<0.05），与给药72 h无统计学差异（P>0.05）。三黄煎剂能够诱导MCF-7、MDA-MB-231细胞凋亡，并上调c-PARP、c-Caspase 3、Bax蛋白的表达，下调Bcl-2蛋白的表达，呈浓度梯度依赖。三黄煎剂能够下调Aurora A蛋白及mRNA的表达、上调p53蛋白及mRNA的表达。结论三黄煎剂能够通过下调Aurora A蛋白及mRNA的表达，抑制Aurora A的生物活性，抑制MCF-7、MDA-MB-231细胞增殖，诱导其凋亡。
- 三黄煎剂 /
- Aurora A /
- 乳腺癌细胞 /
- 凋亡
Abstract: OBJECTIVE To explore the effect of San Huang decoction on the apoptosis of breast cancer cells and the effect on the mRNA and protein expression and function of Aurora kinase A and discuss the underlying mechanism of San Huang induced apoptosis. METHODS The inhibition of breast cancer cells proliferation was determined by CCK-8 assay. The apoptosis of breast cancer cells was detected by AnnexinV-FITC/PI Staining. The expression of mRNA of Aurora A was examined by q-PCR analysis. The expression of apoptosis-related proteins and Aurora A were determined by Western Blot analysis. RESULTS San Huang decoction inhibited the proliferation of breast cancer cells in a does-dependent manner(P<0.05). The effect of inhibition caused by San Huang decoction 48 hours after delivering to breast cancer cells was better than 24 hours(P<0.05) ， although similar as 72 hours(P>0.05). San Huang decoction was also found to induce apoptosis in both MCF-7 and MDA-MB-231 cell lines in a dose-dependent manner. Consistent with cellular results， San Huang decoction treatment significantly increased the apoptosis-related protein level of cleaved-PARP(c-PARP)， cleaved-Caspase 3(c-Caspase 3) and Bax， down-regulated Bcl-2 in a does-dependent manner. Meanwhile， San Huang decoction decreased the mRNA and protein level of Aurora A and increased those of p53 in a does-dependent manner. CONCLUSION San Huang decoction at the first time was able to promote the apoptosis of breast cancer cells via inducing the suppression of Aurora A.
- San Huang decoction /
- Aurora A /
- breast cancer cells /
- apoptosis

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参考文献(24)

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三黄煎剂调节Aurora激酶A促进乳腺癌细胞凋亡的实验研究

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San Huang Decoction Promotes Apoptosis of Breast Cancer Cells Through Regulating Aurora Kinase A

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