UPLC法测定复方虎黄喷剂中虎杖苷、黄芩苷、欧前胡素
Simultaneous Determination of three components in Compound Hu-huang Spray by UPLC
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摘要: 目的 利用梯度洗脱，建立同时测定复方虎黄喷剂中3种指标成分虎杖苷、黄芩苷和欧前胡素的UPLC检测方法。方法 采用UPLC法，Acquity UPLC BEH C18色谱柱（100 mm×2.1 mm，1.7 μm），流动相：乙腈（A）和0.05%磷酸水溶液（B）梯度洗脱；流速：0.4 mL/min；检测波长：280 nm；柱温：35 ℃。结果 虎杖苷的线性范围为0.59～59.0 mg/L (r=0.999 2)，平均回收率为100.6%，RSD为1.56%；黄芩苷的线性范围为1.128～112.8 mg/L (r=0.999 9)，平均回收率为101.5%，RSD为1.68%；欧前胡素的线性范围为0.109～10.9 mg/L (r=0.999 9)，平均回收率为100.6%，RSD为2.75%。结论 本方法操作简便，测定结果准确可靠，可用于复方虎黄喷剂的质量控制。Abstract: OBJECTIVE To develop a quantitative method simultaneously to determinate polydatin and baicalin in compound Hu-huang Spray by UPLC. METHODS The seperation was performed on a column Acquity UPLC BEH C18(100 mm×2.1 mm，1.7 μm). The mobile phase was composed of acetonitrile (A) and 0.05% H3PO4(B) with gradient elution. The flow rate was 0.4 mL /min with the wave length at 280 nm. The temperature was 35℃. RESULTS For polydatin， the calibration curve was linear (r=0.9992) in the range of 0.59~59.0 mg/L， the average recovery was 100.6% (RSD=1.56%). For baicalin， the calibration curve was linear (r=0.9999) in the range of 1.128～112.8 mg/L， the average recovery was 101.5% (RSD=1.68%). For imperatorin， the calibration curve was linear (r=0.999 9) in the range of 0.135～13.5 mg/L， the average recovery was 100.2% (RSD=3.03%). CONCLUSION The method was sensitive， accurate and can be used in the quality control of compound Hu-huang Spray.
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