四角蛤蜊、菲律宾蛤仔及文蛤水提醇沉上清液中相关核苷类物质的含量测定

Content Determination of Major Nucleosides in Supernatant of Mactra veneriformis， Ruditapes philippinarum， and Meretrix meretrix Linnaeus by Ethanol Subsiding Method

    摘要
  • 摘要: 目的 建立四角蛤蜊、菲律宾蛤仔及文蛤软体水提醇沉上清液中主要核苷类成分尿苷、黄嘌呤、胸腺嘧啶、次黄嘌呤、肌苷、鸟苷、2-脱氧鸟苷、2-脱氧肌苷、胸苷、腺苷的测定方法。方法 采用xBridge C18(4.6 mm×250 mm，5 μm)色谱柱；以水和甲醇作为流动相梯度洗脱，以254 nm为检测波长，体积流量0.5 mL/min，柱温：25 ℃。结果 四角蛤蜊、文蛤、菲律宾蛤仔的水提醇沉上清液中含有尿苷、黄嘌呤、胸腺嘧啶、次黄嘌呤、肌苷、鸟苷、2-脱氧鸟苷、2-脱氧肌苷、胸苷、腺苷。结论 本检验方法简便、准确、重现性好，可作为相关蛤蜊软体水提醇沉上清液中主要核苷类成分含量的常规检测方法。

     

    Abstract: OBJECTIVE To establish a method for determining uridine， xanthine， thymine， hypoxanthine， inosine， guanosine， 2-deoxyguanosine， 2-deoxy inosine， thymidine， adenosine in the supernatant of Mactra veneriformis， Ruditapes philippinarum， and Meretrix meretrix Linnaeus by ethanol subsiding method. METHODS The xBridge C18 analytical column(4.6 mm×250 mm，5 μm) was used and the mobile phases were consisted of water and methanol with gradient elution. The flow rate was 0.5 mL/min， column temperature was at 25 ℃， and the wave length was 254 nm. RESULTS The supernatant of Mactra veneriformis， Ruditapes philippinarum and Meretrix meretrix Linnaeus by ethanol subsiding method contains uridine， xanthine， thymine， hypoxanthine， inosine， guanosine， 2-deoxyguanosine， 2-deoxy inosine， thymidine， and adenosine. CONCLUSION This method is easy to practice and accurate and can be used for determining the main nucleoside of supernatant by the ethanol subsiding method for clams.

     

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