Abstract: OBJECTIVE To study the effect of Yishen Huayun method on the intervention mechanism of fibroblast proliferation of chronic pelvic inflammatory process. METHODS Different concentrations of YSHY， YS， HY were used in the incubated NIH/3T3 cells and MTT method was used to detect the cell proliferation. And the effect of different prescriptions for bFGF、TGF-β、HOP expression were observed. RESULTS YISHEN Huayun effectively inhibit the expression of fibroblast proliferation and cytokine， and reduce the production of HOP， which is in a dose-dependent manner.Compared with Yishen Huayu method， the inhibitory effect of Yishen method is relatively low; compared with Yinshen Huayu method， Huayu method achieved low effects， but is more useful than Yishen method. CONCLUSION Yishen Huayu method is effective in inhibiting the fibroblast proliferation in treating chronic pelvic inflammatory disease by inhibiting the expression of cytokines related to fibroblast proliferation and the expression of certain collagen in the extracellular matrix. Compared with the Yinshen method and Huayu method， the combined formula can achieve the best effect.