Abstract: OBJECTIVE To investigate the Xijiao Dihuang Decoction on mRNA expression of autophagy related Atg-5、Beclin-1 protein of the rats' brain tissues， which are endotoxin induced persistent middle cerebral artery occlusion (pMCAO) and to observe the anti-body expression of LC-3 through transmission electron microscope. METHODS Dived the rats into different groups after modeling， and then use different dose of Xijiao Dihuang Decoction for intervention. Extract RNA and use PCR to detect the gene expression of Rat-atg-5 and Beclin -1. The antibody expression of stained LC-3 was observed through transmission electron microscope. RESULTS Atg-5， Beclin-1 and mRNA significantly increased in "Stasis heat" syndrome rat model of "pMCAO" and the model group. The expression was significantly reduced Xijiao Dihuang Decoction middle dose group and positive drug group compared with the model group. LC-3 antibody staining granules suggested that the blank group did not have obvious expression. The model group rats， especially in the sixth day， the expression of model group was significantly increased and positive drug group significantly inhibited LC-3 expression. The third and sixth day reached the best point. Low dose group showed no inhibitory effect. The middle dose group had better effect than high dose group and reached the best point on the sixth day. CONCLUSION Xijiao Dihuang Decoction can protect the brain by significantly reducing the expression of Atg-5， Beclin-1 mRNA and inhibiting the expression of LC-3.