生地黄-山茱萸对AGEs致HUVEC细胞损伤的保护作用
The Protective Effect of Radix Rehmanniae and Cornus Officinalis on HUVEC Stimulated by AGEs
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摘要: 目的 观察生地黄、山茱萸对晚期糖基化终产物（AGEs）作用下人脐静脉内皮细胞（HUVEC）的保护作用。方法 体外培养HUVEC，将细胞分为正常对照组、AGEs组、生地黄组、山茱萸组、生地黄-山茱萸分煎及合煎组，加入终浓度为0.125、0.062 5、0.031 25 g/L的药物预孵1 h后，加入200 mg/L的AGEs作为刺激物，继续培养24 h后，用CCK-8测定并计算药物对AGEs致HUVEC损伤的保护率；取各组细胞上清液，试剂盒测定超氧化物歧化酶（SOD）、丙二醛（MDA）、一氧化氮（NO）、乳酸脱氢酶（LDH），ELISA法测定肿瘤坏死因子-α（TNF-α）、IL-1β。结果 生地黄、山茱萸、生地黄-山茱萸（0.125、0.062 5、0.031 25 g/L）对AGEs致HUVEC损伤均有保护作用。AGEs组细胞上清液中SOD活性、NO含量降低（P<0.05~0.01），MDA、LDH含量增加（P<0.05~0.01），TNF-α、IL-1β分泌增加（P<0.05~0.01）。而各给药组能提高SOD活性、NO含量，降低LDH、MDA，减少TNF-α和IL-1β分泌，与AGEs组比较有显著性差异（P<0.05~0.01）。结论 生地黄、山茱萸、生地黄-山茱萸通过增加内皮细胞NO生成量、抑制氧化应激、调节细胞分泌功能来保护血管内皮细胞的，尤以生地黄-山茱萸保护HUNEC最为明显。Abstract: OBJECTIVE To detect the protective effect of Radix Rehmanniae and Cornus Officinalis on HUVEC stimulated by advanced glycation endoproducts (AGEs).METHODS The cultured HUVEC cells were randomly divided into normal， AGEs， Radix Rehmanniae， Cornus Officinalis， Radix Rehmanniae-Cornus Officinalis separated and combined decoction groups. Firstly， adding 0.125 g/L，0.062 5 g/L，0.031 25 g/L final concentration drugs in advance for 1 h. Secondly， using 200 mg/L AGEs as stimulus to continue culturing for 24 h. Thirdly， using CCK-8 to measure and calculate the protection rate of drugs on HUVEC cells stimulated by AGEs. Last， measuring SOD， MDA， NO， LDH， TNF-α， IL-1β by ELISA method. RESULTS Different density of Radix Rehmanniae， Cornus Officinalis， and Radix Rehmanniae combining Cornus Officinalis all have protective effect on HUVEC cells stimulated by AGEs. The SOD， NO decreased (P<0.05~0.01)， MDA， LDH， TNF-α， IL-1β increased (P<0.05~0.01) in the supernate of AGEs group. Medication administration groups can increase SOD， NO， decrease MDA， LDH， TNF-α， IL-1β， and have a significant difference compared to AGEs group (P<0.05~0.01).CONCLUSION Radix Rehmanniae， Cornus Officinalis， and Radix Rehmanniae combining Cornus Officinalis can protect vascular endothelial cells by increasing production of NO， inhibiting oxidative stress， and regulating emiocytosis. Especially the Radix Rehmanniae combining Cornus Officinalis group has a more obvious effect.
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