Abstract: OBJECTIVE To observe the influence of puerarin on glucose and lipid metabolism in gestational diabetic mellitus(GDM) rats. METHODS High-fat feeding pregnant wistar rats were given streptozocin （STZ） 25 mg/kg intraperitoneally on the first day of pregnant. After injection for 72 hours， rats with FBG between 6.67-16.67 mmol/L were gestational diabetic models， which were randomly divided into two groups: GDM model group(MG， using 0.9% NaCl)， puerain treated group(TG， using puerain 0.25 g/(kg·d)， 6 rats in each group. And we selected 6 pregnant rats as normal gestation group(NG， using 0.9% NaCl). The rats were given drugs by intragastric administration until the offspring were born. The levels of FBG， insulin， serum lipid， TNF-α， IL-6， leptin and adiponection were observed at the age of 25 weeks. Leptin receptor insulin receptor， TNF-α， IL-6， adiponection and leptin mRNA expression in fat and liver tissues were detected by qPCR. RESULTS Compared with GDM model group， the levels of FBG， insulin， TG， TC， LDL-C， TNF-α， IL-6 and leptin of puerarin treated group were significantly decreased(P<0.05， P<0.01). The levels of HDL-C and adiponection of puerarin treated group were significantly increased(P<0.05， P<0.01). The mRNA expression of IR in TG was higher than MG， and the mRNA expression of TNF-α and leptin in TG were decreased significantly （P<0.05）. CONCLUSION Puerarin can improve glucose and lipid metabolism in gestational diabetic rats， the mechanism may be related to the regulation of TNF-α， IL-6， leptin and adiponectin levels.