Abstract: OBJECTIVE To explore the effect of Wenyun mixture and bran stir-baked Atractylodis water extract on Spleen-deficiency Diarrhea in mice. METHODS The spleen deficiency mouse models were established by extract of Sennae Folium. Fecal short chain fatty acids (SCFAs) levels were detected and measured after treatment of different doses of Wenyun mixture and bran stir-baked Atractylodis water extract. RESULTS Compared with the model group， propionic acid level in mice feces was increased in low dose of bran fried Atractylodes water extract group significantly， propionic acid and isovaleric acid level in mice feces were increased in high dose of bran fried Atractylodes water extract group significantly， acetic acid， propionic acid and butyric acid level in mice feces were increased in high dose of Wenyun mixture group significantly. CONCLUSION Wenyun mixture and bran fried Atractylodes water extract could obviously improve short chain fatty acid level to transform spleen Qi and stop diarrhea.