消癌解毒方通过调控PI3K/AKT通路抑制乳腺癌细胞株MDA-MB-231增殖的作用与机制探讨
The Anti-Proliferative Activity and Mechanism Exploration of Xiao'ai Jiedu Decoction on Breast Cancer Cell Lines MDA-MB-231 Through PI3K/AKT Pathway
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摘要: 研究消癌解毒方抗乳腺癌细胞的活性及作用机制。方法 显微镜观察MDA-MB-231细胞形态，MTT法考察细胞的增殖抑制率，流式细胞术观察细胞周期，Western blot检测PI3K/AKT信号通路相关蛋白P53、P21、Cyclin B1、CDK1的表达。结果 与对照组相比，消癌解毒方给药组细胞减少，排列疏松，体积变小，形态变圆；消癌解毒方可以提高MDA-MB-231细胞的增殖抑制率（P<0.05~0.01），使肿瘤细胞的细胞周期阻滞于G2/M期（P<0.05~0.01），可以抑制与细胞周期相关的PI3K/AKT信号通路，上调P53、P21，下调Cyclin B1、CDK1蛋白的表达（P<0.05~0.01）。结论 消癌解毒方能阻滞MDA-MB-231细胞周期，抑制乳腺癌细胞的增殖，其机制可能与抑制PI3K/AKT信号通路有关。Abstract: OBJECTIVE To study the anti-proliferative activity and mechanism of Xiao'ai Jiedu decoction against breast cancer cell lines MDA-MB-231. METHODS The cell morphology was observed by microscope. MTT method was used to investigate the inhibitory rate of Xiao'ai Jiedu decoction against breast cancer cell lines MDA-MB-231. Flow cytometry analyzed the cell cycle distribution. Western blot detected the expression of Cyclin B1 and CDK1 and the expression of upstream signaling molecules P53， P21 in PI3K/AKT signaling pathway. RESULTS Compared with the control group， MDA-MB-231 cells of Xiao'ai Jiedu decoction group showed number-decreased， loosely arranged， morphology-smaller and rounder. Xiao'ai Jiedu decoction inhibited the proliferation of MDA-MB-231 cells（P<0.05， P<0.01）. Xiao'ai Jiedu decoction down-regulated the expression of Cyclin B1 and CDK1， inhibited the PI3K/AKT signaling pathway， and up-regulated the expression of P53 and P21（P<0.05， P<0.01）. CONCLUSION Xiao'ai Jiedu decoction can inhibit the proliferation of MDA-MB-231 breast cancer cells， perhaps related to inhibiting the PI3K/AKT signaling pathway.
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