Abstract: OBJECTIVE To study the effects of Gubi recipe on the PI3K/AKt pathway and synovial tissue of nnee joint in the rats model of knee osteoarthritis. METHODS SD rats(n=40) were duplicated by Hulth's technique and randomly divided into the model group， the Glucosamine Sulfate capsules group， the low dose group of Gubi recipe， the dose group of Gubi recipe and the high dose group of Gubi recipe. Also， the rats in the sham group (n=8) were exposed to the cruciate ligament after the operation， and the normal group (n=8) did not need any disposal. All rats were given the solution by filling the stomach for 8 weeks. 8 weeks later， the the synovial membrane was taken from each rat for observing; Western blot technic of articular cartilage PI3K/AKt pathway AKt activation extent and detect the expression of Bcl-2 and Bax. RESULTS Gubi recipe can improve the knee synovial membrane injury score of rats， effectively reduce the inflammatory reaction in knee synovial membrane. The expression of AKt and downstream target protein Bcl-2 was lower than that of normal group， the expression level of AKt in the high dose group of Gubi recipe was much higher than that in the normal group(P<0.01)， the expression level of AKt in the middle dose group was close to that in the normal group; compared with the model group， the expression of AKt and the downstream target protein Bcl-2 in the high dose group were significantly higher (P<0.01)， and the expression of Bax was decreased. CONCLUSION Gubi recipe can effectively reduce the chondrocyte apoptosis of osteoarthritis of the knee lesions in the process， to some extent improve the pathology of knee osteoarthritis， the mechanism may be related to the intervention of PI3K/AKt pathway of knee osteoarthritis cartilage tissue of rat.