双参平肺方中药味配伍对知母芒果苷提取率的影响与成分作用探讨
Study on Different Compatibility of Shuangshen Pingfei Decoction Influence on Decocting Rate of Mangiferin in Anemarrhena asphodeloides
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摘要: 目的 探究双参平肺方中不同配伍对知母芒果苷提取率的影响，并以成分配伍研究影响复方提取率的作用机理。方法 根据处方中药含有成分的类别进行处方拆方、配伍，分为知母单煎组、处方合煎组、知母-皂苷类中药复方组、知母-酚酸类中药复方组、知母-碱基类中药复方组。以知母中芒果苷提取率为指标，分析配伍对芒果苷提取率的影响；根据拆方结果，对显著影响芒果苷提取率的桑白皮，以其所含的生物碱成分1-脱氧野尻霉素（1-Deoxynojirimycin，DNJ）、氨基酸等代表性成分进行不同配伍，考察桑白皮中成分对知母中芒果苷的影响，探讨芒果苷提取率的影响机理。结果 知母单煎、处方合煎、知母-皂苷类中药复方、知母-酚酸类中药复方、知母-碱基类中药复方配伍的提取率分别为97.33%、47.03%、94.08%、57.53%、34.31%；进一步拆分表明，处方中桑白皮与知母配伍时对芒果苷的提取率影响最为明显，其中桑白皮生物碱部位、DNJ、甘氨酸、β-丙氨酸溶液分别与知母合煎，芒果苷的提取率分别为16.64%、28.13%、40.75%、48.56%。结论 双参平肺方煎煮提取时主要是桑白皮、地骨皮影响了芒果苷的提取率，推测桑白皮中生物碱及氨基酸与芒果苷形成缔合物，团聚分子量增大，影响了芒果苷煎出时的成分溶出。Abstract: OBJECTIVE To explore the influence of different compatibility of Shuangshen Pingfei decoction on the decocting rate of mangiferin in Anemarrhena asphodeloides， and ingredients to research influence on the mechanism of the solution rate. METHODS To disassemble formulas on the basis of the prescription of traditional Chinese medicine containing categories of ingredients. With decocting rate of mangiferin in Anemarrhena asphodeloides Bge as indexes， analyse different compatibility influence on mangiferin decocting rate. Based on these results of disassembled formulas， the representative alkaloid 1-Deoxynojirimycinand amino acid in different compatibility are used to research the influence of compositions of white mulberry on the decocting rate of mangiferin in Anemarrhena asphodeloides Bge and discuss the influence of the decocting rate mechanism with Anion and cation exchange resin processing of samples. RESULTS Research findings shows that the decocting rates of mangiferin in Anemarrhena asphodeloides Bge decoction， prescription combined decoction， Anemarrhena asphodeloides Bge-saponins from Chinese herbal compound， Anemarrhena asphodeloides Bge-phenolic acid from Chinese herbal compound are 97.33%， 47.03%， 94.08%， 57.53%， 34.31%， respectively; Further study shows that Morus alba L. combined with Anemarrhena asphodeloides Bge have the most obvious effect on extraction yield of mangiferin. Anemarrhena asphodeloides Bge decoting with total alkaloids of Morus alba L， DNJ， glycine， β-alanine， respectively. The decocting rate of mangiferin in each group was 16.64%， 28.13%， 40.75% and 48.56%， respectively. CONCLUSION Our results suggest that the decocting rates of mangiferin are mainly influenced by Morus alba L. We also conjectured that alkaloids and amino acids of Morus alba L. form complexes with mangiferin.
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