艾灸调控IL-17对实验性RA大鼠血清RANKL和TGF-β含量的影响

Adjustment of Moxibustion on Serum Level of IL-17， RANKL and TGF-β in RA Rats

    摘要
  • 摘要: 目的 探索艾灸治疗类风湿性关节炎（RA）抗炎的效应及预防骨破坏的IL-17信号转导机制。方法 雄性SD大鼠66只，随机分为空白组、模型组、空白艾灸组、艾灸组、IL-17过表达模型艾灸组、IL-17沉默模型艾灸组、IL-17过表达模型组、IL-17沉默模型组，前6组每组各9只，后2组每组各6只。大鼠右后足足垫部皮内注入弗氏完全佐剂0.15mL制作RA模型。造模成功后第2天进行慢病毒注射建立IL-17基因过表达、IL-17基因沉默模型。RA造模后第7天开始麦粒灸大鼠肾俞、足三里2穴各5壮，每日1次，治疗18d。观测计算大鼠足跖肿胀度、热痛域；采用ELISA法检测大鼠血清IL-17、RANKL、TGF-β含量。结果 造模后大鼠右后足容积显著高于空白组(P<0.01)，热痛域值显著低于空白组（P<0.01）；艾灸后大鼠右后足容积显著低于模型组(P<0.01)，热痛域显著高于模型组（P<0.01）。模型组大鼠血清IL-17、RANKL显著高于空白组，血清TGF-β含量显著低于空白组(P<0.01)；艾灸后艾灸组大鼠血清IL-17、RANKL显著低于模型组，血清TGF-β含量显著高于模型组（P<0.01）。IL-17过表达模型艾灸组大鼠RANKL含量显著高于艾灸组（P<0.01）；IL-17过表达模型艾灸组大鼠TGF-β含量显著低于艾灸组(P<0.01)。IL-17沉默艾灸组大鼠RANKL含量显著低于艾灸组和空白组(P<0.01)。结论 艾灸可能通过调控IL-17，从而调节RA大鼠血清骨破坏因子RANKL以及骨保护因子TGF-β含量，起到抑制RA滑膜炎症后期骨破坏、保护骨组织的作用。

     

    Abstract: OBJECTIVE To explore the IL-17 signal transduction mechanism of anti-inflammatory effect of moxibustion on RA rats. METHODS 66 SD rats were randomly assigned to control， model， control+moxa， moxa， IL-17 overexpression+moxa， IL-17 knockdown+moxa， IL-17 overexpression and IL-17 knockdown groups. Complete freund's adjuvant were injected into the right paw of SD rats to establish RA model， and siRNAs were injected for IL-17 overexpression/knockdown models. After 7d， moxibustion treatments were performed for 18d. RESULTS The swelling degree of RA rats and the serum level of IL-17 and RANKL were significantly higher than control group(P<0.01)， and the PWTL of RA rats，serum level of TGF-β were significantly lower than control group. After 18d of moxibustion treatment， the swelling degree of RA rats and the serum level of IL-17，RANKL were significantly lower than control group(P<0.01)，and the PWTL of RA rats and serum level of TGF-β were significantly higher than control group. In addition， the serum level of RANKL in IL-17 knockdown+moxa rats were significantly higher than moxa group(P<0.01); the serum level of RANKL in IL-17 knockdown+moxa rats were significantly lower than moxa group and control group（P<0.01）. CONCLUSION Moxibustion may adjust the serum level of RANKL and TGF-β by supressed the serum level of IL-17 therefore relieving RA in RA rats.

     

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