6种宁神类药物抗抑郁作用动物实验研究
Animal Experimental Study on the Antidepressant Effects of Six Tranquilizing Medicines
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摘要: 目的 探讨石菖蒲、酸枣仁、朱砂、远志、茯神、合欢花等6种宁神类药物的抗抑郁作用。方法 采用旷场试验评价药物对小鼠自发活动的影响，强迫游泳和强迫悬尾实验评价药物对小鼠不动时间的影响，Western-blot免疫印迹检测胶质纤维酸性蛋白的表达。结果 与对照组比，旷场试验中各实验组动物活动总路径差异无统计学意义（P>0.05），强迫游泳实验中石菖蒲、朱砂、远志、茯神、合欢花组不动时间显著缩（P<0.05~0.01），悬尾实验中石菖蒲、朱砂、远志和茯神组不动时间显著缩短（P<0.05~0.01），各组GFAP蛋白表达差异无统计学意义（P>0.05）。结论 石菖蒲、朱砂、远志、茯神、合欢花具有抗抑郁作用。Abstract: OBJECTIVE To explore the antidepressant effect six tranquilizing medicines， namely Acorus tatarinowii， Ziziphus jujuba， Zinnober， Thinleaf Milkwort Root-bark， Poria cum Radix， and Silktree Albizziae Flower. METHODS The effects of six medicines on spontaneous activity of mice were evaluated by field test and the effects on immobility time of mice were evaluated by forced swimming test and tail suspension test. The expression of glial fibrillary acidic protein was detected by western blot. RESULTS Compared with control group， no significant difference was found in total animal activities path in field test for each group (P>0.05). However， in the forced swimming test and tail suspension test， the immobility time of mice of Acorus tatarinowii， Zinnober， Thinleaf Milkwort Root-bark， Poria cum Radix， and Silktree Albizziae Flower group was both significantly reduced (P<0.05~0.01). There was no significant difference in expression of GFAP protein for each group (P>0.05). CONCLUSION Acorus tatarinowii， Zinnober， Thinleaf Milkwort Root-bark and Poria cum Radix have antidepressant effect.
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