地耳草黄酮类成分UPLC-MS分析
Analytical Study on the Flavonoids Constituents in Herba Hyperici Japonici by UPLC-MS
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摘要: 目的 建立超高效液相色谱-质谱仪联用技术（UPLC-MS）对地耳草中黄酮类成分分析的方法。方法 采用Alltima C18色谱柱(5 μm，250 mm×4.6 mm，i.d.)，以乙腈-0.8%醋酸为流动相，梯度洗脱，流速为0.8 mL/min，检测波长为254 nm，柱温为30 ℃。在电喷雾负离子模式下监测采集数据。结果 初步鉴定8种黄酮类化合物，并同时测定其中6种黄酮的含量。6种黄酮类化合物的浓度与峰面积的线性关系良好(r2>0.998 2)；加样回收率为97.6%~99.7%。结论 该方法准确可靠，重现性好，可作为地耳草内在质量评价的依据。Abstract: OBJECTIVE To establish an Ultra Performance Liquid Chromatography (UPLC-MS) method to analyze the flavonoids constituents in Herba Hyperici Japonici.METHODS The analysis was performed on an Alltima C18 chromatographic column with a gradient solvent system of acetonitrile-0.8% acetic acid at a flow rate of 0.8 mL/min. The detection wavelength was 254 nm and the column temperature was 30 ℃. The data was collected under the negative electrospray ion mode.RESULTS 8 flavonoids constituents being separated and identified， six of them were detected simultaneously. The result shows the detected flavonoids constituents have good linearity between concentration and peak area (r2>0.998 2)， and the average recoveries of the method were between 97.6%~99.7%.CONCLUSION Being simple， accurate and reproducible， UPLC-MS can be used for evaluating the quality of Herba Hyperici Japonici.
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Keywords:
- UPLC-MS /
- Herba Hyperici Japonici /
- flavonoids constituents
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