Abstract: OBJECTIVE To analyze and compare the different biomarker groups of liver-fire hyperactivity syndrome and yin-deficiency and yang-hyperactivity syndrome of hypertension and explore molecular basis of TCM syndrome of hypertension. METHODS The gas chromatography-time of flight mass spectrography (GC-TOFMS) technology was employed to analyze the serum metabonomics. RESULTS ①Glutamic oxalacetic transaminase (GOT) and triacylglycerol in group of yin-deficiency and yang-hyperactivity syndrome of hypertension is markedly higher than the group of liver-fire hyperactivity syndrome (P<0.05). ②Groups of liver-fire hyperactivity syndrome and yin-deficiency and yang-hyperactivity syndrome in hypertension are different in the principal component scores three-dimensional diagram. The different compounds which can be identified include citrate， oxalate， dioxide heptanoic acid， glycerate， soft hydrochloric acid， ethanol amine， inositol， oleic acid， stearic acid， aspartate， threose acid， palmitic acid. CONCLUTION Metabonomics method can distinguish group of liver-fire hyperactivity syndrome of hypertension and group of yin-deficiency and yang-hyperactivity syndrome of hypertension. The difference of biomarker does exist in the two syndromes and is expected to provide a certain objective basis for TCM syndrome differentiation typing of hypertension.