Abstract: OBJECTIVE To evaluate the ACE-inhibitory and antioxidant activities of Mactra veneriformis enzymatic hydrolysis peptides. METHODS Pepsin and trypsin were used to simulate enzymatic process in vivo，and then enzymatic hydrolysis peptides of Mactra veneriformis were obtained. The ACE inhibitory activity of Mactra veneriformis enzymatic hydrolysis peptides was measured by high performance liquid chromatography， and the radical scavenging effect was detected by Fenton system. RESULTS Mactra veneriformis enzymatic hydrolysis peptides was obtained by enzymatic hydrolysis of trypsin for 6h，which ACE-inhibitory activity of enzymatic hydrolysis peptides is the best， IC₅₀=15.06 μg/mL. The best rate of hydroxyl radical scavenging of Mactra veneriformis enzymatic hydrolysis peptides is 93.78%，which was obtained by trypsin used for 2 h.CONCLUSION Mactra veneriformis enzymatic hydrolysis peptides have a definite ACE-inhibitory and antioxidant activity.