Abstract: OBJECTIVE To investigate the effect of the combined administration of herbal pair Euphorbia Pekinensis (DJ) and Glycyrrhiza glabra (GC) at different doses or ratios on hepatocellular carcinoma (HCC) ascites in mice. METHODS A total of 120 male Kunming mice were randomly divided into normal control， model， DJ-GC groups ratios 1∶0.5， 1∶1， 1∶2， 1∶4， DJ-alone group and GC-alone group. HCC ascites model was constructed by inoculating H22 cells intraperitoneally. HCC ascites mice in the drug treatment groups were intragastrically administrated with corresponding doses of Euphorbia Pekinensis and Glycyrrhiza glabra for 12d， respectively. Then， the therapeutic effects of herbal pair DJ and GC acting on HCC acites was analyzed in terms of body weight， ascites volume， abdominal circumference and the index of liver and kidney. After that， the expressions of AQP2 and V2R were detected by Western Blot， and the level of ALT， AST， BUN， CR were detected by biochemical method. RESULTS When compared with model group， the DJ-alone group and DJ-GC 1∶2 group could efficiently reduced the ascites volume， abdominal， body weight (P<0.05~0.01) and the expression of aquaporin-2 and renal vasopressin receptors-2 in the kidney tissues (P<0.05~0.01). DJ-GC 1∶2 group could enhance the effect of DJ-alone group ( P<0.05~0.01 )， while the DJ-GC 1∶4 group could reduce the effect of DJ-alone group ( P<0.05~0.01 ). CONCLUSION The therapeutic effects of DJ on HCC were altered in accordance with its combination with GC at different ratios by being enhanced or weakened， which might be partially related to their regulation on aquaporin-2 and renal vasopressin receptors-2 signal axis.