Abstract: OBJECTIVE To explore the pathogenic factors， diagnosis and treatment strategy and preventive actions via performing a retrospective analysis of 128 operative cases with cervical intraepithelial neoplasia(CIN) due to damp-heat pathogen. METHODS Clinical data of the 128 cases hospitalized for CIN surgery due to damp-heat from January， 2007 to July， 2016， including the symptoms， signs， testing indexes， choice of operation， pathological results as well as relevant pathogenic factors， were recorded on the same chart and analyzed. RESULTS The disease was more likely to happen to females in their 30s to 50s， mainly manifested as the abnormal vaginal discharge， postcoital bleeding， menstrual disorder， accounting for 75%. It is generally classified as the type of damp-heat， accompanied with kidney deficiency， spleen deficiency， liver stagnation and blood stasis after syndrome differentiation in traditional Chinese medicine. 77.3% of the patients experienced cervical hypertrophy together with cervical erosion according to the tests， while still 10.2% of them had no abnormal appearance. The positive result of human papilloma virus (HPV) was mainly seen as 16 / 18， while its quantitative value had little to do with the with the severity of disease. 50.8% of the patients got the same pathogenic results before and after surgery， 29.7% experienced an exacerbation after operation and 19.5% experienced an improvement. The discovery rate of carcinoma in situ or early invasive carcinoma after surgery went up by 5.4% than before. CONCLUSION CIN due to damp-heat has certain clinical specificities， whose occurrence is correlated to HPV infection arising from decreased immune function after premature sexual life， multiple sexual partners and chronic inflammation of cervix and vagina. Cervical cold knife conization has advantages in avoiding the misdiagnosis， missed diagnosis， postoperative massive hemorrhage and interferences of pathologic diagnosis from operative thermoelectric effects. It is therefore vital to recognize the special nature of the disease， choose the proper operation and pay attention to holistic therapy to avoid the reoccurrence.